AOAC-RI ERP Book MICRO Sept 2016.pdf

Document 1 of 2 – 3M Petrifilm Salmonella Express PTM Study Report

Evaluation of the 3M ™ Petrifilm™ Salmonella Express System for the Detection of Salmonella in Select Foods

AOAC Research Institute Performance Tested Method

Abstract The 3M™ Petrifilm™ Salmonella Express SALX System is used for the rapid and specific detection and l biochemical confirmation of Salmonella spp. from enriched food matrices. The 3M Petrifilm SALX System was compared to the USDA/FSIS-MLG 4.05 Isolation and Identification of Salmonella from Meat, Poultry, Pasteurized Egg and Catfish Products for raw ground chicken, pasteurized liquid whole egg, raw ground beef, raw ground pork, and cooked chicken nuggets and to FDA/BAM Chapter 5 Salmonella for frozen uncooked shrimp, fresh bunched spinach, dry dog food, and stainless steel. Twenty (20) replicates of each matrix were analyzed at one inoculum level: 0.2-2 CFU/test portion. Five (5) control replicates were also analyzed at 0 CFU/test portion (un-inoculated). Results were analyzed by both the Mantel-Haenszel chi square for unpaired test portions and by the Probability of Detection (POD). There were no statistically significant differences in the number of positive samples detected and confirmed by the 3M Petrifilm SALX System and the reference methods for the nine matrices analyzed. Lot-to-lot assay variability, shelf-life stability, and assay ruggedness parameters (primary enrichment incubation time, incubation time of the 3M™ Petrifilm™ Salmonella Plate, incubation temperature of the 3M™ Petrifilm™ Salmonella Plate, incubation temperature of the 3M™ Petrifilm™ Salmonella Disk, incubation time of the 3M™ Petrifilm™ Salmonella Disk, and various plate rehydration options were evaluated and found no unexpected results. The 3M™ Petrifilm™ SALX System demonstrated reliability as a rapid qualitative method for the detection and biochemical confirmation of Salmonella in a wide variety of foods.

Method Author Robert P. Jechorek

3M Food Safety Department 3M Center - Bldg 260-6B-01 St. Paul, MN 55144-1000

Submitting Company 3M Company

Food Safety Department 3M Center, Bldg. 275-5W-05

Page 1 of 41 NOTE: The official document is electronic. Paper copies are uncontrolled.