AOAC-RI ERP Book MICRO Sept 2016.pdf

February 20, 2013

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• Biochemically confirmed positive results:

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Colonies that are green to blue, blue to dark blue, or black or have a blue precipitate around them are

biochemically confirmed positive for Salmonella species.

• Biochemically confirmed negative results:

Colonies that remain the same red, dark red or brown color without a blue precipitate are negative for

Salmonella species.

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6. Colonies may be subcultured for further identification. When subculturing, wear appropriate protective

apparel and follow standard good laboratory safety practices (GLP) 2 .

a. With a gloved hand, lift the top film and aseptically remove the colony either from the gel or the 3M Petrifilm SALX Confirmation Disk. If a 3M Petrifilm SALX Confirmation Disk is covering the gel, aseptically peel the disk away and then aseptically remove the colony from the gel. b. Streak the colony onto/into media per appropriate reference method 5,6,7 . NOTE: If the colonies cannot be subcultured within 1 hour of plate removal from the incubator, then store the 3M Petrifilm SALX Plates for later analysis by placing in a sealed plastic bag at -20 to -10°C for no longer than 72 hours in the dark. Allow 3M Petrifilm SALX Plates to warm to room temperature (20-

25°C / <60% RH) before continuing subculturing for identification.

7. After the test is complete, dispose of the 3M Petrifilm SALX Plates and 3M Petrifilm SALX Confirmation

Disks in accordance with current industry standards and/or local regulations.

Validation Study

This study was conducted according to AOAC guidelines outlined in the AOAC General Referee approved harmonized PTM/OMA validation protocol, “Validation Outline for 3M™ Petrifilm™ Salmonella Express

System.”

Inclusivity Testing Methodology

Inclusivity testing was conducted to ensure that the 3M Petrifilm SALX System can detect many different serovars of Salmonella . One hundred four (104) pure cultures (Table 2 in Appendix) were

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