AOAC-RI ERP Book MICRO Sept 2016.pdf

February 20, 2013

Raw Ground Beef 1 For raw ground beef test portions, there were 13 confirmed positives the 3M Petrifilm SALX method at all 2 four time points and 13 confirmed positives for the USDA/FSIS-MLG method. All un-inoculated control 3 samples were negative by both methods. A χ 2 value of 0.0 was obtained between the reference and 4 candidate method using both the traditional and alternative confirmation procedures, indicating no 5 significant difference between the candidate and reference methods at any of the four time points. 8 obtained between the reference and candidate method samples confirmed using either the traditional or 9 alternative confirmation procedures at all four time points. The confidence intervals obtained for dPOD C 10 indicated no significant difference between the two methods. A dPOD CP value of 0.00 with 95% confidence 11 intervals of (-0.28, 0.28) was obtained between presumptive and confirmed results using either 12 confirmation procedure for all four time points. The confidence intervals obtained for dPOD CP indicated no 13 significant difference between the presumptive and confirmed results at all four time points. 6 7 For the low level inoculum, a dPOD C value of 0.00 with 95% confidence intervals of (-0.28, 0.28) was For raw ground pork test portions, there were 9 confirmed positives by the 3M Petrifilm SALX System at all four time points and 9 confirmed positives by the USDA/FSIS-MLG method. All un-inoculated control samples were negative by both methods. A χ 2 value of 0.0 was obtained between the reference and candidate method samples using both the traditional and alternative confirmation procedures, indicating no significant difference between the candidate and reference methods at any of the four time points. obtained between the reference method and candidate method samples confirmed using either the traditional or alternative confirmation procedure at all four time points. The confidence intervals obtained confidence intervals of (-0.28, 0.28) was obtained between presumptive and confirmed results using either the traditional or alternative confirmation procedure for all four time points. The confidence intervals indicated no significant difference between the presumptive and confirmed results at for dPOD C indicated no significant difference between the two methods. A dPOD CP value of 0.00 with 95% For the low level inoculum, a dPOD C value of 0.00 with 95% confidence intervals of (-0.28, 0.28) was For pasteurized liquid whole egg test portions, there were 6 confirmed positives by the 3M Petrifilm SALX method at both time points and 4 confirmed positives by the USDA/FSIS-MLG method. All un-inoculated control samples were negative for both methods. A χ 2 value of 0.98 was obtained between the USDA/FSIS- MLG and candidate method using both traditional and alternative confirmation procedures, indicating no significant difference between the candidate and reference method at any of the time points. obtained between the USDA/FSIS-MLG method and the candidate method samples confirmed using either the traditional or alternative confirmation procedure at both the 18 hour and 24 hour primary enrichment ISO 6579 method and the candidate method samples confirmed using either the traditional or alternative confirmation procedure at both the 18 hour and 24 hour primary enrichment time points. The confidence time points. A dPOD C value of 0.05 with 95% confidence intervals of (-0.22, 0.31) was obtained between the For the low level inoculum, a dPOD C value of 0.10 with 95% confidence intervals of (-0.17, 0.35) was 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 Raw Ground Pork obtained for dPOD CP all four time points. Low Microbial Foods Pasteurized Liquid Whole Eggs

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