AOAC-RI ERP Book MICRO Sept 2016.pdf

February 20, 2013

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values indicated no significant difference between the candidate and value of 0.00 with 95% confidence intervals of (-0.27, 0.27) were obtained

intervals obtained for the dPOD C reference method. A dPOD CP

between presumptive and confirmed results using either the traditional or alternative confirmatory procedure at both the 18 hour and 24 hour primary enrichment time points. The confidence intervals

obtained for dPOD CP

indicated no significant difference between the presumptive and confirmed results.

Dry Dog Food 7 For dry dog food test portions, there were 16 confirmed positives by the 3M Petrifilm SALX System at both 8 the 18 hour and 24 hour primary enrichment time points and 14 confirmed positives the FDA/BAM 9 method. All un-inoculated control samples were negative for both methods. A χ 2 value of 0.52 was 10 obtained between the reference and candidate method using both the alternative and traditional 11 confirmation procedures, indicating no significant difference between the candidate and reference 12 methods at either of the time points.

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For the low level inoculum, a dPOD C

value of 0.10 with 95% confidence intervals of (-0.17, 0.35) was

obtained between the reference method and candidate method samples confirmed following either the traditional or alternative confirmation procedure at both the 18 hour and 24 hour primary enrichment time

points. The confidence intervals obtained for dPOD C

indicated no significant difference between the two

methods. A dPOD CP

value of 0.00 with 95% confidence intervals of (-0.25, 0.25) was obtained between

presumptive and confirmed results using either the traditional or alternative confirmation procedure for both the 18 hour and 24 hour primary enrichment time points. The confidence intervals obtained for dPOD CP indicated no significant difference between the presumptive and confirmed results.

Stainless Steel Environmental Surface

For stainless steel environmental surfaces, there were 6 confirmed positives for the 3M Petrifilm SALX System at both the 18 hour and 24 hour primary enrichment time points and 7 confirmed positives for the FDA/BAM method. All un-inoculated control samples were negative for both methods. A χ 2 value of 0.11 was obtained between the reference and candidate method using both the traditional and alternative confirmation procedures, indicating no significant difference between the candidate and reference

methods at any of the time points.

For the low level inoculum, a dPOD C

value of -0.05 with 95% confidence intervals of (-0.32, 0.23) was

obtained between the reference method and candidate method samples confirmed using either the traditional or alternative confirmation at both 18 hour and 24 hour primary enrichment time points. The dPOD CP value of 0.00 with 95% confidence intervals of (-0.27, 0.27) was obtained between presumptive and confirmed results using either the traditional or alternative confirmation procedure for both the 18 hour and 24 hour primary enrichment time points. The confidence intervals obtained for dPOD CP indicated no confidence intervals obtained for dPOD C indicated no significant difference between the two methods. A

significant difference between the presumptive and confirmed results.

Cooked Chicken Nuggets

For cooked chicken nugget test portions, there were 6 confirmed positives by the 3M Petrifilm SALX method at both the 18 hour and 24 hour primary enrichment time points and 6 confirmed positives the USDA/FSIS-MLG method. All un-inoculated control samples were negative for both methods. A χ 2 value of 0.0 was obtained between the reference and candidate method using both the traditional and alternative confirmation procedures, indicating no significant difference between the candidate and reference

methods at any of the time points.

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