AOAC-RI ERP Book MICRO Sept 2016.pdf

February 20, 2013

Figure 3. 3M™ Petrifilm™ Salmonella Express vs. FDA-BAM – stainless steel 1 Surface

Sample surface with swab or sponge and store in DE broth 2hr at room temp

3M

BAM

1 sponge + 225ml Lactose broth, Mix well and let stand 60±5 min at room temp Mix and adjust pH to 6.8±0.2 if necessary 24±2h h at 35°C

1 sponge + 225ml 3M BPW ISO (prewarmed to 42C)

Homogenize 2 min 18-24h h at 42±1°C

0.1ml sample + 10ml RV, 24±2h at 42±0.2°C

1.0ml sample + 10ml TT, 24±2h at 35±2°C

0.1ml sample + 10ml RV, 24±2h at 42±0.2°C

1.0ml sample + 10ml TT, 24±2h at 35±2°C

Run the 3M Petrifilm Salmonella Express System

• Streak to XLD & HE - 24±2h at 35°C • Streak to BS - incubate at 35°C for 24±2h,

Pick typical colony and transfer to TSI/LIA slants 24±2h at 35°C

• Streak to XLD & HE - 24±2h at 35°C • Streak to BS - incubate at 35°C for 24±2h, read and pick if applicable. Re-incubate another 24 h

read and pick if applicable. Re-incubate another 24 h

Transfer typical colonies to TSI/LIA slants 24±2h at 35°C

Biochemical/serological confirmation on all enrichments according to FDA-BAM

Transfer typical colonies to TSI/LIA slants 24±2h at 35°C

Biochemical/serological confirmation on all enrichments according to FDA-BAM

Biochemical/serological confirmation on all enrichments according to FDA-BAM

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