AOAC-RI ERP Book MICRO Sept 2016.pdf

Document 2 of 2 – 3M Petrifilm Salmonella Express PTM Study Report

Table 11. 3M Petrifilm SALX Method Comparison Chi-Square Results for Raw Ground Chicken vs USDA/FSIS MLG (Internal Data) 1

3M Petrifilm SALX Method

Presumptive positive test portions on 3M Petrifilm SLAX Plates

Confirmed

X 2 Alternative Confirmation

X 2 Traditional Confirmation

SALX Confirmation Disk Positive Reference

APC CFU/g MPN/25g*

USDA/FSIS MLG

Inoculation Level

Inoculating Organism

Total Samples

18 Hr Primary Enrichment – 8 Hr RV-R10 2 o Enrichment

Control

N/A

< 0.075

5

0

0

0

0

-

-

S. enterica ser. Heidelberg

7.1 x 10 3

0.2-2 CFU/

20

15

15

14

14

0.12

0

1.15

Test Portion NCTC 5717

(0.70-2.07)

18 Hr Primary Enrichment – 24 Hr RV-R10 2 o Enrichment

Control

N/A

< 0.075

-

5

0

0

0

0

-

S. enterica ser. Heidelberg

7.1 x 10 3

20

15

15

14

14

0.12

0

0.2-2 CFU/

1.15

Test Portion NCTC 5717

(0.70-2.07)

24 Hr Primary Enrichment – 8 Hr RV-R10 2 o Enrichment

Control

N/A

< 0.075

-

5

0

0

0

0

-

S. enterica ser. Heidelberg

7.1 x 10 3

20

15

15

14

14

0.12

0

0.2-2 CFU/

1.15

Test Portion NCTC 5717

(0.70-2.07)

24 Hr Primary Enrichment – 8 Hr RV-R10 2 o Enrichment

Control

N/A

< 0.075

-

5

0

0

0

0

-

S. enterica ser. Heidelberg

7.1 x 10 3

20

15

15

14

14

0.12

0

0.2-2 CFU/

1.15

Test Portion NCTC 5717

(0.70-2.07)

2 3

*Includes 95% confidence interval