AOAC-RI ERP Book MICRO Sept 2016.pdf

Document 2 of 2 – 3M Petrifilm Salmonella Express PTM Study Report

Table 21. 3M Petrifilm SALX Method Comparison POD Results for Raw Frozen Shrimp (Internal Data) 1

3M Petrifilm SALX Method

Reference Method

x c

Strain MPN a /25g

N b

95% CI g

e

d

dPOD CP f

95% CI

95% CI

x

Matrix

18 Hr Primary Enrichment – Time Point

POD CC

POD CP

Frozen Uncooked Shrimp -

0

5

0

0

0.00 - 0.43

0

0

0.00 - 0.43

0 -0.43 – 0.43

Salmonella

8 Hr 2 0 RV-R10 Enrichment Virchow

0.77 (0.41- 1.36)

20

12

0.6 0.39 – 0.78 12

0.6 0.39 – 0.78

0 -0.28 – 0.28

Confirmed by the 3M Confirmation Disk

18 Hr Primary Enrichment – 24 Hr 2 0 RV-R10 Enrichment 24 Hr Primary Enrichment – 8 Hr 2 0 RV- R10 Enrichment 24 Hr Primary Enrichment – 24 Hr 2 0 RV-R10 Enrichment 18 Hr Primary Enrichment – 18 Hr Primary Enrichment – 24 Hr 2 0 RV-R10 Enrichment 24 Hr Primary Enrichment – 8 Hr 2 0 RV-R10 Enrichment 24 Hr Primary Enrichment – 24 Hr 2 0 RV-R10 Enrichment

0

5

0

0

0.00 - 0.43

0

0

0.00 - 0.43

0 -0.43 – 0.43

ATCC

0.77 (0.41- 1.36)

20

12

0.6 0.39 – 0.78 12

0.6 0.39 – 0.78

0 -0.28 – 0.28

51955

0

5

0

0

0.00 - 0.43

0

0

0.00 - 0.43

0 -0.43 – 0.43

0.77 (0.41- 1.36)

20

12

0.6 0.39 – 0.78 12

0.6 0.39 – 0.78

0 -0.28 – 0.28

0

5

0

0

0.00 - 0.43

0

0

0.00 - 0.43

0 -0.43 – 0.43

0.77 (0.41- 1.36)

20

12

0.6 0.39 – 0.78 12

0.6 0.39 – 0.78

0 -0.28 – 0.28

Frozen Uncooked Shrimp -

0

5

0

0

0.00 - 0.43

0

0

0.00 - 0.43

0 -0.43 – 0.43

Salmonella

8 Hr 2 0 RV-R10 Enrichment Virchow

0.77 (0.41- 1.36)

20

12

0.6 0.39 – 0.78 12

0.6 0.39 – 0.78

0 -0.28 – 0.28

Confirmed by the Reference Method

0

5

0

0

0.00 - 0.43

0

0

0.00 - 0.43

0 -0.43 – 0.43

ATCC

0.77 (0.41- 1.36)

20

12

0.6 0.39 – 0.78 12

0.6 0.39 – 0.78

0 -0.28 – 0.28

51955

0

5

0

0

0.00 - 0.43

0

0

0.00 - 0.43

0 -0.43 – 0.43

0.77 (0.41- 1.36)

20

12

0.6 0.39 – 0.78 12

0.6 0.39 – 0.78

0 -0.28 – 0.28

0

5

0

0

0.00 - 0.43

0

0

0.00 - 0.43

0 -0.43 – 0.43

0.77 (0.41- 1.36)

20

12

0.6 0.39 – 0.78 12

0.6 0.39 – 0.78

0 -0.28 – 0.28

a MPN = Most Probable Number is based on the POD of reference method test portions across labs using the AOAC MPN calculator with 95% confidence interval b N = Number of test potions c x = Number of positive test portions

d POD C = Candidate method confirmed positive outcomes divided by the total number of trials e POD R = Reference method confirmed positive outcomes divided by the total number of trials f dPOD C = Difference between the candidate method confirmed results and candidate method confirmed result POD values g 95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level

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