AOAC-RI ERP Book MICRO Sept 2016.pdf

Document 2 of 2 – 3M Petrifilm Salmonella Express PTM Study Report

Table 27. 3M Petrifilm SALX Method Comparison POD Results for Cooked Chicken Nuggets (Internal Data) 1

3M Petrifilm SALX Method

Reference Method

X c

Strain MPN a /325g

N b

95% CI g

d

e

95% CI

x

95% CI

dPOD C f

Matrix

Time Point

POD R

POD C

5

0

0

0.00 - 0.43

0

0

0.00 - 0.43

0 -0.43 – 0.43

Salmonella

0

18 Hr Primary Enrichment 24 Hr Primary Enrichment

0.03 (0.01- 0.08)

20

6

0.3 0.15 – 0.52

6

0.3 0.15 – 0.52

0 -0.27 – 0.27

Typhimurium

Cooked Chicken Nuggets Vs. USDA/FSIS- MLG

5

0

0

0.00 - 0.43

0

0

0.00 - 0.43

0 -0.43 – 0.43

ATCC

0

0.03 (0.01- 0.08)

20

6

0.3 0.15 – 0.52

6

0.3 0.15 – 0.52

0 -0.27 – 0.27

14028

a MPN = Most Probable Number is based on the POD of reference method test portions across labs using the AOAC MPN calculator with 95% confidence interval b N = Number of test potions c x = Number of positive test portions d POD C = Candidate method confirmed positive outcomes divided by the total number of trials e POD R = Reference method confirmed positive outcomes divided by the total number of trials f dPOD C = Difference between the candidate method confirmed results and candidate method confirmed result POD values

g 95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level

2 3 4 5 6 7 8 9

10 11 12 13 14 15 16 17