AOAC-RI ERP Book MICRO Sept 2016.pdf

OMAMAN-08/Collaborative Study Protocol March 2014 Expert Review Panel Use Only

3M Petrifilm SALX Collaborative Study

July 2013

OMA-2013-July-XXX

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for Use.

The method was awarded PTM certification number 061301 on June 5, 2013.

1.3

Study Director for Collaborative Study

The co-Study Directors for this collaborative study:

Robert P. Jechorek

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3M Food Safety Department 3M Center - Bldg 260-6B-01 St. Paul, MN 55144-1000 Email: rpjechorek@mmm.com

Phone: 651-733-9764

Co-SD: Erin Crowley and Patrick Bird

Q Laboratories, Inc. Cincinnati, OH 45214

Email: e crowley@qlaboratories.com

pbird@qlaboratories.com

Phone: 513-471-1300

2.0

Collaborators

A total of 15-16 collaborators will be solicited to participate in this study. A minimum of 10 laboratories, with acceptable data, will be needed for the successful completion of this study. Qualified laboratories, familiar with Petrifilm, will act as collaborators. All collaborators have been, or will be, thoroughly trained by a 3M representative on the use of Petrifilm.

A sample letter to collaborators can be found in Appendix 8.1. This letter will be sent to each

collaborator prior to the start of the study.

3.0

Collaborative Study Design

AOAC Research Institute Ex ert Review Panel Use Only

3.1 The laboratory conducting the study will prepare the test portions. In this study, raw fresh ground beef (25g) and dry dog food (375g) will be analyzed for Salmonella following the 3M Petrifilm SALX protocol and appropriate reference method. The USDA- FSIS MLG Ch. 4.06 method will be used as the reference method for ground beef and Each week, collaborators will receive 72 samples of a test matrix for analysis: 12 uninoculated controls, 12 low level (target 0.2 – 2.0 cfu/test portion), and 12 high level (target 2 – 5 cfu/test portion) for each method - SALX and reference method. See flow diagrams 8.5 and 8.6 for the study details. Each laboratory will test all test portions. Each test matrix will be inoculated with a different Salmonella spp . as listed in Table 1, FDA/BAM Chapter 5 will be used as the reference method for dry dog food. 3.2

randomized and blind-coded for analysis.

Table 1. Overview of Study Design

DRAFT DOCUMENT

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