AOAC-RI ERP Book MICRO Sept 2016.pdf

OMAMAN-08/Collaborative Study Protocol March 2014 Expert Review Panel Use Only

3M Petrifilm SALX Collaborative Study

July 2013

OMA-2013-July-XXX

1

# Test Portions (per method)

Inoculating organism

Target Salmonella Levels

Test Portion Codes

Reference Method

MATRIX

0 cfu/test portion

12

S. enterica subsp. Ohio

Raw ground beef (80/20)

USDA/FSIS MLG Ch. 4.06

Random number between 100-199

0.2-2 cfu/test portion

12

25g-SALX 25g-MLG

STS 81 (Fresh)

2-5 cfu/test portion

12

0 cfu/test portion

12

Salmonella enterica ser. Poona ATCC 4840

Dry dog food 375g-SALX 25g-BAM

Random number between 200-299

FDA/BAM Chapter 5

0.2-2 cfu/test portion

12

2-5 cfu/test portion

12

2 3 4 5 6 7 8 9

3.3

Study Schedule

3.3.1 One matrix will be tested each week of the study. All data should be sent to the

SD by the 4 th week of study.

3.3.2 The contract laboratory will prepare the test portions and ship the test portions refrigerated via overnight courier to the collaborators to arrive on a Friday. Test portion processing and analysis will begin on Monday. Data forms will be completed and faxed or emailed back to the co-Study Director by the following

10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33

Friday.

4.0

Test Portion Preparation

4.1 Fresh raw ground beef and dry dog food will be obtained from local retail outlets and screened for the presence of Salmonella . Naturally contaminated test portions will be used if available at sufficient levels. Alternatively, the test portions will be artificially inoculated to meet the targets of 0.2-2.0 cfu/test portion for the low and 2 – 5 cfu/test portion for the high with the appropriate concentration of a wet inoculum for raw

AOAC Research Institute Expert Review Panel Use Only

ground beef and a lyophilized inoculum for dry dog food.

Preparation of test portions

4.2

4.2.1 Ground beef: A liquid inoculum will be added drop-wise to a bulk quantity of ground beef for each inoculation level - 0.2-2 cfu/25g and 2-5 cfu/25g. The bulk quantity will then be mixed to achieve equal distribution of analyte throughout. The test portions will be stored at 2-8 ° C for 72-96 h prior to testing. 4.2.2 Dry dog food : A wet inoculums in pellet form will be added to a bulk quantity of dry dog food for each inoculation level - 0.2-2 cfu/25g and 2-5 cfu/25g. The bulk quantity will then be mixed to achieve equal distribution of analyte throughout. The test portions will be stored at room temperature for 2 weeks prior to testing. Samples should be checked during the stabilization period to ensure

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