AOAC-RI ERP Book MICRO Sept 2016.pdf
OMAMAN-08/Collaborative Study Protocol March 2014 Expert Review Panel Use Only
3M Petrifilm SALX Collaborative Study
July 2013
OMA-2013-July-XXX
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For the detection of Salmonella species in a Select Foods, Dry Dog Food, and Stainless
Steel.
The 3M Petrifilm SALX Plate is a sample ready-to-use chromogenic culture medium system that contains a cold-water-soluble gelling agent and is selective and differential for Salmonella , providing a presumptive result. The 3M Petrifilm SALX Confirmation Disk contains a biochemical substrate that facilitates the biochemical confirmation of
Salmonella organisms.
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Test Kit information
5.2
5.2.1 3M Petrifilm Salmonella Express Plate :
5.2.1.1 6536 - Petrifilm Salmonella Express Plate 25 plates/pouch; 2
pouches/box
5.2.1.2 6537 - Petrifilm Salmonella Express Plate 25 plates/pouch; 8
pouches/case
5.2.2 Typical Formula
5.2.2.1 Magnesium chloride (anhydrous)
13.4g
5.2.2.2 Sodium chloride 5.2.2.3 Casein peptone
7.2g
4.54g 1.45g 0.036g
5.2.2.4 Monopotassium phosphate
5.2.2.5 Malachite green 5.2.2.6 Demineralized water 5.2.2.7 pH 5.1 ± 0.2 at 25°C
1000 mL
5.2.2.8 Adjust pH as required to meet performance standards.
5.2.3 3M Petrifilm Salmonella Confirmation Disk:
5.2.3.1 6538 - Petrifilm Salmonella Confirmation Disk - 5 disks/pouch; 1
pouch/box
5.2.3.2 6539 - Petrifilm Salmonella Confirmation Disk - 5 disks/pouch; 5
pouches /case
Reagents
5.3
5.3.1 3M Salmonella Enrichment Broth: SEB500 - 500g/bottle, SEB025 - 2.5kg/bottle 5.3.2 3M Salmonella Enrichment Supplement: SESUP001 - 1.0g/vial
AOAC Research Institute Expert Review Panel Use Only
5.3.3 3M Rappaport-Vassiliadis R10 (R-VR10) broth (BP0288500)
Selective agar
5.4
5.4.1 Hektoen Agar 5.4.2 XLD Agar 5.4.3 XLT4 Agar
5.4.4 Bismuth Sulfite (Wilson-Blair Agar)
Formatted: Font: Italic
5.4.5 Brilliant Green Sulfa Agar
Apparatus
5.5
5.5.1 Sterile 10µL inoculation loop. - for streaking enrichments onto the SALX
plate
5.5.2 Permanent ultra fine tip marker. – for circling presumptive positive colonies
on the SALX plate
DRAFT DOCUMENT
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