AOAC-RI ERP Book MICRO Sept 2016.pdf

OMAMAN-08/Collaborative Study Protocol March 2014 Expert Review Panel Use Only

3M Petrifilm SALX Collaborative Study

July 2013

OMA-2013-July-XXX

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For the detection of Salmonella species in a Select Foods, Dry Dog Food, and Stainless

Steel.

The 3M Petrifilm SALX Plate is a sample ready-to-use chromogenic culture medium system that contains a cold-water-soluble gelling agent and is selective and differential for Salmonella , providing a presumptive result. The 3M Petrifilm SALX Confirmation Disk contains a biochemical substrate that facilitates the biochemical confirmation of

Salmonella organisms.

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Test Kit information

5.2

5.2.1 3M Petrifilm Salmonella Express Plate :

5.2.1.1 6536 - Petrifilm Salmonella Express Plate 25 plates/pouch; 2

pouches/box

5.2.1.2 6537 - Petrifilm Salmonella Express Plate 25 plates/pouch; 8

pouches/case

5.2.2 Typical Formula

5.2.2.1 Magnesium chloride (anhydrous)

13.4g

5.2.2.2 Sodium chloride 5.2.2.3 Casein peptone

7.2g

4.54g 1.45g 0.036g

5.2.2.4 Monopotassium phosphate

5.2.2.5 Malachite green 5.2.2.6 Demineralized water 5.2.2.7 pH 5.1 ± 0.2 at 25°C

1000 mL

5.2.2.8 Adjust pH as required to meet performance standards.

5.2.3 3M Petrifilm Salmonella Confirmation Disk:

5.2.3.1 6538 - Petrifilm Salmonella Confirmation Disk - 5 disks/pouch; 1

pouch/box

5.2.3.2 6539 - Petrifilm Salmonella Confirmation Disk - 5 disks/pouch; 5

pouches /case

Reagents

5.3

5.3.1 3M Salmonella Enrichment Broth: SEB500 - 500g/bottle, SEB025 - 2.5kg/bottle 5.3.2 3M Salmonella Enrichment Supplement: SESUP001 - 1.0g/vial

AOAC Research Institute Expert Review Panel Use Only

5.3.3 3M Rappaport-Vassiliadis R10 (R-VR10) broth (BP0288500)

Selective agar

5.4

5.4.1 Hektoen Agar 5.4.2 XLD Agar 5.4.3 XLT4 Agar

5.4.4 Bismuth Sulfite (Wilson-Blair Agar)

Formatted: Font: Italic

5.4.5 Brilliant Green Sulfa Agar

Apparatus

5.5

5.5.1 Sterile 10µL inoculation loop. - for streaking enrichments onto the SALX

plate

5.5.2 Permanent ultra fine tip marker. – for circling presumptive positive colonies

on the SALX plate

DRAFT DOCUMENT

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