AOAC-RI ERP Book MICRO Sept 2016.pdf

OMAMAN-08/Collaborative Study Protocol March 2014 Expert Review Panel Use Only

3M Petrifilm SALX Collaborative Study

July 2013

OMA-2013-July-XXX

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5.11.7 Colonies may be subcultured for further identification. When subculturing, wear appropriate protective apparel and follow standard good laboratory safety 5.11.8 With a gloved hand, lift the top film and aseptically remove the colony either from the gel or the 3M Petrifilm SALX Confirmation Disk. If a 3M Petrifilm SALX Confirmation Disk is covering the gel, aseptically peel the disk away and then 5.11.9 Streak the colony onto/into media per appropriate reference method. 5.11.10 NOTE: If the colonies cannot be subcultured within 1 hour of plate removal from the incubator, then store the 3M Petrifilm SALX Plates for later analysis by placing in a sealed plastic bag at -20 to -10°C for no longer than 72 hours in the dark. Allow 3M Petrifilm SALX Plates to warm to room temperature (20-25°C / 5.11.11 After the test is complete, dispose of the 3M Petrifilm SALX Plates and 3M Petrifilm SALX Confirmation Disks in accordance with current industry standards practices (GLP). aseptically remove the colony from the gel. <60% RH) before continuing subculturing for identification.

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and/or local regulations.

6.0

Reporting Raw Data

6.1

Report data using the data report form in Appendix 8.2.

6.2 Upon completion of each food type, the laboratory will fax or email the completed data

form to the Study Director.

6.3

Copies of all related test results (data sheets and confirmation results) should be

retained by the collaborating labs for a minimum of one year.

7.0

Analyzing Raw Data

The resulting data will be analyzed by two different calculations: probability of detection (POD)

and relative limit of detection (RLOD).

7.1 Probability of Detection (POD) - POD is the proportion of positive analytical outcomes for a qualitative method for a given matrix at a given analyte level or concentration. POD is concentration dependent. Please see the revised microbiology guidelines 1 for

7.1.1.1 Estimate of repeatability - standard deviation (s r ) 7.1.1.2 Determine POD values for each matrix and concentration - the number AOAC Research Institute Expert Review Panel Use Only

complete analysis information on POD calculations.

7.1.1 Calculate:

of positive outcomes divided by the total number of trials.

7.1.1.3 Estimate of reproducibility – standard deviation of the laboratory POD

values (s POD

) and confidence intervals.

1 AOAC INTERNATIONAL Methods Committee Guidelines for Validation of Microbiological Methods for Food and Environmental Surfaces (pre-publication document, 2012) Sharon Brunelle, Robert LaBudde, Maria Nelson, and Paul Wehling

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