AOAC-RI ERP Book MICRO Sept 2016.pdf

OMAMAN-08/Collaborative Study Protocol March 2014 Expert Review Panel Use Only

3M Petrifilm SALX Collaborative Study

July 2013

OMA-2013-July-XXX

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Appendix 8.4

Study Materials

Materials provided by collaborator 4 Sterile 10µL inoculation loop. - for streaking enrichments onto the SALX plate 5 Permanent ultra fine tip marker. – for circling presumptive positive colonies on the SALX plate 6 Sterile serological pipettes. – capable of 1mL 7 Pipettes . – capable of 100µL 8 Sterile pipette tips. – capable of 100µL 9 Stomacher® . – Seward or equivalent 10 Filter Stomacher® bags . - Seward or equivalent 11 Incubators . – capable of maintaining 41.5° ± 1°C 12 Incubator -capable of maintaining 35°C± 1°C 13 Waterbath - capable of maintaining 42°C± 0.2°C(circulating, thermostatically controlled) 14 Freezer . – capable of maintaining -10° to -20°C, for storing opened SALX pouches , hydrated SALX 15 plates, SALX plates after incubation 16 Refrigerator . – capable of maintaining 2° - 8°C, for storing unopened 3M SALX plates and SALX disks 17 Sterile distilled water

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Materials and reagents provided by the study sponsor

3M Petrifilm Salmonella Express Plate 3M Petrifilm Salmonella Confirmation Disk 3M Salmonella Enrichment Broth 3M Salmonella Enrichment Supplement 3M Rappaport-Vassiliadis R10 (R-VR10) broth

3M Petrifilm Flat Spreader

Enrichment

Buffered Peptone Water

Lactose Broth

mRV

TT broth (Hajna formulation)

AOAC Research Institute Expert Review Panel Use Only

Selective agar

Hektoen Agar - BAM

XLD Agar - BAM

BS - BAM

XLT4 Agar - MLG

BGS - MLG

Confirmation

TSI LIA

API 20E or VITEK

O and H serotyping supplies

DRAFT DOCUMENT

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