AOAC-RI ERP Book MICRO Sept 2016.pdf

OMAMAN-08/Collaborative Study Protocol March 2014 Expert Review Panel Use Only

3M Petrifilm SALX Collaborative Study

July 2013

OMA-2013-July-XXX

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Appendix 8.6 – Flow Diagram for Dry Dog Food

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Dry dog food

3M

BAM

375g + 3375mL 3M Salmonella Enrichment + Supplement (prewarmed to 41.5C)

25g + 225ml Lactose broth, Blend 2 min and let stand 60±5 min at room temp Mix and adjust pH to 6.8±0.2 if necessary 24±2h h at 35°C

Homogenize 2 min 18-24h at 41.5±1°C

Reference Method Confirmation

3M SALX Method Confirmation

3M SALX Method

0.1ml sample + 10ml RV, 24±2h at 42±0.2°C in circulating waterbath

0.1ml sample + 10ml RV, 24±2h at 42±0.2°C in

1.0ml sample + 10ml TT, 24±2h at 35±2°C

1.0ml sample + 10ml TT, 24±2h at 35±2°C

Hydrate SALX plate with 2.0 mL DI water, close top film gently, use 3M spreader to distribute diluent evenly, let sit on bench at least 1 hr prior to use, protected from light

Place room temperature SALX confirmation disk onto SALX plate, under top film and incubate 41.5±1°C for 4-5 h

circulating waterbath

Record confirmed + results: green to blue, blue to dark blue or black or have a blue precipitate around

Streak to XLD, HE, BS - incubate according to specific plate instructions

Streak to XLD, HE, BS- incubate according to specific plate instructions

Streak enriched sample onto gel of SALX plate. Close topfilm and incubate 41.5±1°C for 24±2 hr.

Transfer typical colonies to TSI/LIA slants 24±2h at 35°C

Transfer typical colonies to TSI/LIA slants 24±2h at 35°C

Transfer typical colonies to TSI/LIA slants 24±2h at 35±1°C

Biochemical/serological confirmation on all enrichments according to FDA-BAM AOAC Research Institute Expert Review Panel Use Only Biochemical/serological confirmation on all enrichments according to FDA-BAM

Circle presumptive Salmonella colonies (red to brown with yellow zone or gas bubble)

Biochemical/serological confirmations on all enrichments according to FDA-BAM

Record sample as positive if typical colonies appear on SALX plate

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DRAFT DOCUMENT

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