AOAC-RI ERP Book Micro Jan 19 2017

OMAMAN-36 E/ AOAC PTM Report ERP Use Only January 2017

Figure7: Summary data: Top: Green channel ( Salmonella detection) Lower: Yellow channel (internal control)

Green all TTP Stability test Salmonella spp .

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Salmonella enteritidis Salmonella abony Citrobacter freundii

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Yellow allTTP Stability test Salmonella spp .

Salmonella enteritidis Salmonella abony Citrobacter freundii

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Figure7 : Ct values from the accelerated aging studies showed no slope over the course of the 120 day accelerated aging study in either the Salmonella (green) detection channel or the internal control (yellow) channel. There was a weak green signal in the C. freundii channel at 120 days, attributed to cross contamination in the culture. (TTP: Test time point) Method developer summary data shelf life QIAsymphony DNA Midi Kit The QIAsymphony Bacteria Kit used in these studies is based on the QIAsymphony DNA Midi Kit. All cartridge chemicals are identical, except for the Proteinase K that is included in the QIAsymphony DNA Midi Kit but omitted from the QIAsymphony Bacteria Kit. Proteinase K is not required for the simple heated lysis implemented in the QIAsymphony Bacteria Kit. It is used in the reference DNA Midi Kit to disrupt the membranes of animal and human cells and tissues. The inclusion of the Proteinase K in the kits for functional testing did not show any changes over the storage of the DNA Midi Kit. One can reasonably assume that the other components of the parent kit (magnetic beads, lysis, binding and elution buffers) that remain constant between the kits would be stable for the same 24 month period. We are providing stability data on the QIAsymphony DNA

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