AOAC RI ERP Final Action Recommendations eBook

FINAL (Version 4)

DuPont™ BAX ® System Real-Time PCR Assay for Salmonella : Collaborative Study

1

Table 5 - BAX® System vs. Reference Method Results for Frankfurters (Low Level Inoculum)

Candidate presumptive (CP)

Candidate confirmed (CC)

Candidate result (C)

Reference method (R)

C vs. R

MPN/ Test Portion

Statistic

Laboratory

dLPOD e (C,R)

dLPOD (CP,CC)

POD(CP) a

POD(CC) b

POD(C) c

POD(R) d

N

x

N

x

N

X

N

X

1 2 3 4 5 6 7 8 9

12 12 12 12 12 12 12 12 12 12 12 12 12 12 NA 168

5 5

0.42 0.42 0.83 0.50 0.58 0.58 0.33 0.50 0.50 0.50 0.67 0.58 0.42 0.42 0.52 0.44 0.60 0.51 0.46 0.52 0.00 0.00 0.16 0.51 0.46 0.52 NA

12 12 12 12 12 12 12 12 12 12 12 12 12 12 NA 168

5 5

0.42 0.42 0.83 0.33 0.58 0.58 0.33 0.50 0.50 0.50 0.67 0.58 0.42 0.42 0.51 0.43 0.58 0.50 0.45 0.52 0.00 0.00 0.17 0.50 0.46 0.52 NA

12 12 12 12 12 12 12 12 12 12 12 12 12 12 NA 168

5 5

0.42 0.42 0.83 0.33 0.58 0.58 0.33 0.50 0.50 0.50 0.67 0.58 0.42 0.42 0.51 0.43 0.58 0.50 0.45 0.52 0.00 0.00 0.17 0.50 0.46 0.52 NA

12 12 12 12 12 12 12 12 12 12 12 12 12 12 NA 168

5 5

0.42 0.42 0.83 0.33 0.58 0.58 0.33 0.50 0.50 0.50 0.67 0.58 0.42 0.42 0.51 0.43 0.58 0.50 0.45 0.52 0.00 0.00 0.17 0.50 0.46 0.52 NA

0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 -0.11 0.11 NA

0.00 0.00 0.00 0.17 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.01 -0.10 0.12 NA

10

10

10

10

6 7 7 4 6 6 6 8 7 5 5

4 7 7 4 6 6 6 8 7 5 5

4 7 7 4 6 6 6 8 7 5 5

4 7 7 4 6 6 6 8 7 5 5

10 f 11 12 13 14 15 All

NA

NA

NA

NA

0.47 0.21 1.04

Estimate

87

85

85

85

LCL UCL

g

s r

LCL UCL

h

s L

LCL UCL

i

s R

UCL LCL

j

P T

0.6831

0.5570

0.5570

0.5570

2

DuPont Nutrition & Health

Page 21