AOAC RI ERP Final Action Recommendations eBook

Dupont BAX Salmonella Pre- Collaborative Study For Expert Review Panel Use Only Do Not Distribute

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Table 3. Sample Analysis with Re-growth

Test from Primary Enrichment

Test from Re-growth

Re-growth Required

Sample Type

Meat and Seafood

Raw ground beef with soy

Yes Yes Yes Yes Yes Yes Yes Yes

No No No No

No No No No No No No No

Beef trim

Frankfurters

Shrimp

Poultry and Eggs

Ground turkey Chicken wings

No No

Dried eggs Shell eggs

Yes Yes

Produce

Frozen peas Orange juice

Yes

Yes Yes

No

No

Yes

Dairy Products

Nonfat dry milk

No

Yes Yes

Yes

Ice cream

Yes

No

Environmentals

Ceramic tile

Yes Yes

Yes Yes

No No

Plastic

Miscellaneous

Peanut butter

Yes

Yes Yes Yes Yes

Yes No*

Cocoa

Yes*

White pepper Infant formula

Yes Yes

No

No * Cocoa samples tested from the primary enrichment were diluted 1:50 in BHI broth before processing.

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Statistical Analysis 3 Data analysis was performed using each of the metrics below according to the format described in the 4 AOAC International Methods Committee Guidelines for Validation of Microbiological Methods for Food 5 and Environmental Surfaces and using the Least Cost Formulations, Ltd., AOAC Binary Data 6 Interlaboratory Study Workbook [6]. 7 For the purposes of this evaluation, the probability of detection (POD) was defined as the number of 8 positive outcomes divided by the total number of trials. POD was estimated with a 95% confidence 9 interval for each of the following levels: 10 • the candidate presumptive results (POD CP ), 11 • the candidate confirmatory results (POD CC ), 12 • the candidate method results based on the presumptive and confirmatory results (POD C ) 13 • the reference method results (POD R ). 14

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