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Table 4. BAX® System Presumptive vs. Confirmed Results – Meat and Seafood

95% CI f

dPOD CP e

BAX® Presumptive

BAX® Confirmed

Test Portions

MPN a

Sample Type

Media

X b POD CP c

95% CI f

X b POD CC d

95% CI f

Control

5

0

0

(0, 0.43)

0

0

(0, 0.43)

0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

(-0.45, 0.45)

Raw ground beef with soy (25 g)

BPW

0.94 (0.68, 1.3)

60

36

0.60

(0.47, 0.71) 36

0.60

(0.47, 0.71) (0.57, 1.0)

(-0.049, 0.049)

8.6 (6.2, 12)

5 5

5 0

1.0

(0.57, 1.0)

5 0

1.0

(-0.45, 0.45) (-0.45, 0.45)

Control

0

(0, 0.43)

0

(0, 0.43)

Raw ground beef with soy (325 g)

mTSB+caa+n

0.94 (0.68, 1.3)

60

39

0.65

(0.52, 0.76) 39

0.65

(0.52, 0.76) (0.57, 1.0)

(-0.049, 0.049)

8.6 (6.2, 12)

5 5

5 0 7 5 0

1.0

(0.57, 1.0)

5 0 7 5 0

1.0

(-0.45, 0.45) (-0.45, 0.45) (-0.14, 0.14) (-0.45, 0.45) (-0.45, 0.45) (-0.14, 0.14) (-0.45, 0.45) (-0.45, 0.45) (-0.14, 0.14) (-0.45, 0.45) (-0.45, 0.45) (-0.14, 0.14) (-0.45, 0.45)

Control

0

(0-0.43)

0

(0, 0.43)

Beef trim (25 g)

BPW

0.85 (0.50, 1.4)

20

0.35

(0.18-0.57) (0.57-1.0)

0.35

(0.18, 0.57) (0.57, 1.0)

4.3 (2.7, 7.0)

5 5

1.0

1.0

Control

0

(0-0.43)

0

(0, 0.43)

Beef trim (325 g)

MP media

0.85 (0.50, 1.4) 4.3 (2.7, 7.0)

20

10

0.50

(0.30, 0.70) 10

0.50

(0.30, 0.70) (0.57, 1.0)

5 5

5 0

1.0

(0.57-1.0)

5 0

1.0

Control

0

(0, 0.43)

0

(0, 0.43)

Frankfurters (325 g)

BPW

0.46 (0.23, 0.93)

20

10

0.50

(0.30, 0.70) 10

0.50

(0.30, 0.70) (0.57, 1.0)

3.2 (1.6, 6.5)

5 5

5 0

1.0

(0.57, 1.0)

5 0

1.0

Control

0

(0, 0.43)

0

(0-0.43)

Shrimp (25 g)

LB

0.71 (0.40, 1.2)

20

11

0.55 0.80

(0.34-0.74) 11

0.55 0.80

(0.34, 0.74) (0.38, 1.0)

2.5 (1.5, 4.2)

5

4

(0.38, 1.0)

4

2 3 4 5 6 7 8

a MPN = Most Probable Number based on the POD of reference method test portions using the AOAC MPN calculator (with 95% confidence interval)

b X = Number of positive test portions

c POD CP = Candidate method presumptive positive outcomes divided by the total number of trials d POD CC = Candidate method confirmed positive outcomes divided by the total number of trials

e dPOD CP = Difference between the candidate method presumptive result and candidate method confirmed result POD values f 95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level

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