AOAC RI ERP Final Action Recommendations eBook
Dupont BAX Salmonella Pre- Collaborative Study For Expert Review Panel Use Only Do Not Distribute
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Table 11. BAX® System vs. Reference Method Results – Dairy Products
95% CI f
e
BAX® Method
Reference Method
dPOD C
Test Portions
MPN a
Sample Type
Media
X b POD C c
95% CI f
X b POD CC d
95% CI f
Control
5
0
0
(0, 0.43)
0
0
(0, 0.43)
0 0 0 0
(-0.45, 0.45) (-0.14, 0.14) (-0.45, 0.45) (-0.45, 0.45) (-0.14, 0.41) (-0.62, 0.27) (-0.45, 0.45) (-0.14, 0.14) (-0.45, 0.45) (-0.45, 0.45) (-0.27, 0.27) (-0.27, 0.62)
Brilliant Green Water / BHI
Nonfat dry milk (25 g)
1.2 (0.79, 1.8) 9.4 (5.7, 16)
20
11
0.55
(0.34, 0.74) 11
0.55
(0.34, 0.74) (0.57, 1.0)
5 5
5 0 9 3 0 6 4 0 6 5
1.0
(0.57, 1.0)
5 0 6 4 0 6 4 0 6 4
1.0
Control
0
(0, 0.43)
0
(0, 0.43)
Ice cream (25 g)
LB
0.31 (0.17, 0.49)
20
0.45 0.60
(0.26, 0.66) (0.23, 0.88)
0.30 0.80
(0.15, 0.52) (0.38, 1.0)
0.15 -0.20
1.8 (1.1, 2.7)
5 5
Control
0
(0, 0.43)
0
(0, 0.43)
0 0 0 0 0
Ice cream (25 g)
BPW
0.31 (0.17, 0.49)
20
0.30 0.80
(0.15, 0.52) (0.38, 1.0)
0.30 0.80
(0.15, 0.52) (0.38, 1.0)
1.8 (1.1, 2.7)
5 5
Control
0
(0, 0.43)
0
(0, 0.43)
Brilliant Green Water
Ice cream (25 g)
0.31 (0.17, 0.49)
20
0.30
(0.15, 0.52) (0.57, 1.0)
0.30 0.80
(0.15, 0.52) (0.38, 1.0)
1.8 (1.1, 2.7)
5
1.0
0.20
2 3 4 5 6 7 8 9
a MPN = Most Probable Number based on the POD of reference method test portions using the AOAC MPN calculator (with 95% confidence interval)
b X = Number of positive test portions
c POD C = Confirmed candidate method positive outcomes divided by the total number of trials d POD R = Confirmed reference method positive outcomes divided by the total number of trials = Difference between the candidate method and reference method POD values e dPOD C
f 95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level
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