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Table 4. Data Summary – Test method vs Reference Method

Collaborative Study Approved Protocol Expert Review Panel Use Only

BAX ® Method

Reference Method

MPN a /Test Portion (cfu / test portion for surface testing) (95% CI)

Enrichment Media/Sample Size/Enrichment Time

dPOD c f

N b

95% CI g

Matrix

Strain

d

e

x c

POD C

95% CI

x

POD R

95% CI

Control

5

0

0

(0, 0.43)

0

0

(0, 0.43)

0

(-0.45, 0.45)

S . Heidelberg DD13017

TSB+n/375g/ 24 hr

Ground Beef

0.37 (0.20, 0.59)

20

5

0.25

(0.11, 0.4)

4

0.20

(0.08, 0.42)

0.05

(-0.21, 0.30)

Control

5

0

0

(0, 0.43)

0

0

(0, 0.43)

0

(-0.45, 0.45)

S . Heidelberg DD13017

Ground Beef

BPW/25g/ 20 hr

0.37 (0.20, 0.59)

20

4

0.2

(0.08, 0.42)

4

0.2

(0.08, 0.42)

0

(-0.14, 0.14)

Chicken Rinse Cream Cheese Cream Cheese

BPW/30ml/16 and 22 hr MP/25g/12 and 24 hr

Naturally Occurring

NA

20

11

0.55

(0.34, 0.74)

11

0.55

(0.34, 0.74)

0

(-0.14, 0.14)

Control

5

0 2 0 5 0 7 5 0 4 5 0 3 5 0 5 0 5

0

(0, 0.43)

0 5 0 5 0 4 5 0 4 5 0 4 5 0 5 0 5

0

(0, 0.43)

0

(-0.45, 0.45) (-0.38, 0.022) (-0.45, 0.45) (-0.14, 0.14) (-0.45, 0.45) (-0.13, 0.40) (-0.45, 0.45) (-0.45, 0.45) (-0.14, 0.14) (-0.45, 0.45) (-0.45, 0.45) (-0.29, 0.19) (-0.45, 0.45) (-0.45, 0.45) (-0.14, 0.14) (-0.45, 0.45) (-0.14, 0.14)

S . Typhimurium DD586 S . Typhimurium DD586

0.20 (0.077, 0.39)

20

0.1

(0.03, 0.30)

0.25

(0.11, 0.47)

-0.15

Control

5

0

(0, 0.43)

0

(0, 0.43)

0 0 0 0 0 0 0 0 0 0 0 0 0

LB/25g/24 hr

0.20 (0.077, 0.39)

20

0.25

(0.11, 0.47)

0.25

(0.11, 0.47)

Control

5

0

(0, 0.43)

0

(0, 0.43)

S . Newport DD1261

MP/25g/12 and 24 hr

Lettuce

0.40 (0.22, 0.64)

20

0.35

(0.18-0.57) (0.57, 1.0)

0.20

(0.08, 0.42) (0.57, 1.0)

0.15

4.3 (2.5, 8.2)

5 5

1.0

1.0

Control

0

(0, 0.43)

0

(0, 0.43)

S . Newport DD1261

Lettuce

LB/25g/24 hr

0.40 (0.22, 0.64)

20

0.2 1.0

(0.08, 0.42) (0.57, 1.0)

0.2 1.0

(0.08, 0.42) (0.57, 1.0)

4.3 (2.5, 8.2)

5 5

Control

0

(0, 0.43)

0

(0, 0.43)

S . Newport DD1261

LB/25g/24 hr + regrowth

Lettuce

0.40 (0.22, 0.64)

20

0.15

(0.05, 0.36) (0.57, 1.0)

0.2 1.0

(0.08, 0.42) (0.57, 1.0)

-0.05

4.3 (2.5, 8.2)

5 5

1.0

Control

0

(0, 0.43)

0

(0, 0.43)

S . Tennessee DD13062 S . Tennessee DD13062

BPW/375g/24 + regrowth LB/375g/24 + regrowth

Dry Pet Food

0.29 (0.13, 0.53)

20

0.25

(0.11, 0.47)

0.25

(0.11, 0.47)

Control

5

0

(0, 0.43)

0

(0, 0.43)

Dry Pet Food

0.29 (0.13, 0.53)

20

0.25

(0.11, 0.47)

0.25

(0.11, 0.47)

22