AOAC RI ERP Final Action Recommendations eBook

Collaborative Study Approved Protocol Expert Review Panel Use Only

regrowth

NA

5 5

5 0

1.0

(0.57, 1.0)

5 0

1.0

(0.57, 1.0)

0 0 0 0 0 0 0 0 0 0 0 0 0 0

(-0.45, 0.45) (-0.45, 0.45) (-0.14, 0.14) (-0.45, 0.45) (-0.45, 0.45) (-0.14, 0.14) (-0.45, 0.45) (-0.45, 0.45) (-0.14, 0.14) (-0.14, 0.14) (-0.45, 0.45) (-0.14, 0.14) (-0.14, 0.14) (-0.14, 0.14)

Control

0

(0, 0.43)

0

(0, 0.43)

LB/4”X4” sponge/18 hr +/- regrowth BPW/4”X4” sponge 18 hr +/- regrowth

S . Mbandaka (DD13240)

Plastic surfaces

NA NA

20

13

0.65

(0.43, 0.82) (0.57, 1.0)

13

0.65

(0.43, 0.82) (0.57, 1.0)

5 5

5 0 6 5 0 9 5 0

1.0

5 0 6 5 0 9 5 0

1.0

Control

0

(0, 0.43)

0

(0, 0.43)

S . Lexington (DD13068)

Tile surfaces

NA NA

20

0.3 1.0

(0.15, 0.52) (0.57, 1.0)

0.3 1.0

(0.15, 0.52) (0.57, 1.0)

5 5

Control

0

(0, 0.43)

0

(0, 0.43)

S . Lexington (DD13068)

LB/4”X4” sponge 18 hr +/- regrowth

Tile surfaces

NA NA

20

0.45

(0.26, 0.66) (0.57, 1.0)

0.45

(0.26, 0.66) (0.57, 1.0)

5 5

1.0

1.0

Control

0

(0, 0.43)

0

(0, 0.43)

S . Newport (DD13079)

White Pepper

1.3 (0.81, 2.2)

20

14

0.7 1.0

(0.48, 0.85) (0.57, 1.0)

14

0.7 1.0

(0.48, 0.85) (0.57, 1.0)

TSB/25 g/22 hrs

26 (20, 44)

5 5

5 0

5 0

Control

0

(0, 0.43)

0

(0, 0.43)

S . Typhimurium (DD1467)

Ground Beef with Soy

MLG mTSB/325g/22 hr

1.0 (0.74, 1.4)

60

39

0.65

(0.52, 0.76)

39

0.65

(0.52, 0.76)

0

(-0.049, 0.049)

6.0 (4.4, 8.4)

5 5

5 0

1.0

(0.57, 1.0)

5 0

1.0

(0.57, 1.0)

0 0

(-0.45, 0.45) (-0.14, 0.14)

Control

0

(0, 0.43)

0

(0, 0.43)

S. Enteritidis (ATCC13706)

Shell Eggs

MP/1000 g/48 hrs

1.6 (0.93, 3.1)

20

17

0.85

(0.64, 0.95)

14

0.70

(0.48, 0.85)

0.15

(-0.11, 0.39)

a MPN = Most Probable Number is based on the POD of reference method test portions across labs using the AOAC MPN calculator, with 95% confidence interval b N = Number of test potions c x = Number of positive test portions d POD C = Candidate method presumptive positive outcomes confirmed positive e POD R = Reference method confirmed positive outcomes divided by the total number of trials f dPOD C = Difference between the candidate method and reference method POD values g 95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level

25