AOAC RI ERP Final Action Recommendations eBook

Collaborative Study Approved Protocol Expert Review Panel Use Only

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10.5

Study Materials

BAX® System Real-Time PCR Assay for Salmonella Collaborative Study Materials

Materials supplied by DuPont

All BAX ® System Real-Time PCR Assay for Salmonella detection kits (includes BAX® System lysis buffer.–Two bottles of 12 mL each, BAX® System protease.–One vial of 400 µL, BAX® System PCR tubes.–12 strips of 8 tubes containing one PCR tablet per tube.), BAX® System Q7 cycler/detector with computer workstation, BAX® System application software and

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BAX ® MP Media

Materials provided by Q Laboratories, Inc., Coordinating Laboratory

1. Standard Methods Agar for APC of test matrices

Enrichment

2. Universal Pre-enrichment broth

3. BPW

4. RV-R10 broth for USDA reference method 5. Individual Ingredients to prepare FDA RV

6. TT Broth (Hajna formulation) for USDA reference method

7. TT Broth for FDA reference method

Selective agar

8. Hektoen Agar

9. XLD Agar

10. Bismuth Sulfite

11. XLT4 Agar

12. Brilliant green sulfa agar

Confirmation

13. TSI 14. LIA 15. BHI

16. API 20E or VITEK GN cards 17. O and H serotyping supplies

Additional Supplies and Reagents required but not provided

18. Pipettes.–For transferring reagents; two adjustable mechanical pipettes covering 20- 200 µ L and 5-50 µ L; one repeating pipette; and one multi-channel pipette covering 8 channels and 5-50 µ L. Pipettes should be calibrated to deliver required volumes within

10%.

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