AOAC RI ERP Final Action Recommendations eBook

Collaborative Study Approved Protocol Expert Review Panel Use Only

19. Pipette tips with barriers.–0.5-250 µ L, 0.5-100 µ L extended barrier; 5 mL repeater

1 2 3 4 5 6 7 8 9

pipette tips.

20. PCR tube holders.–For transferring a rack of tubes from the cooling block to the

cycler/detector.

21. Incubators.–For maintaining enrichment media at 35 ± 1 ° C and 39-42 ° C.

22. Cluster tubes and racks. –For lysis.

23. Capping/de-capping tools.–For removing and sealing cluster tube caps and PCR tube

caps without jarring the contents.

24. Thermal block(s) with inserts.–For maintaining lysis tubes at 37 ± 2°C, 95 ± 3°C, and

10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42

4 ± 3°C.

25. Water bath- Capable of maintaining 42 ± 1°C for secondary selective enrichments. 26. Refrigerator – capable of maintaining 2-8°C, for storing the BAX® System Real-Time PCR

Assay for Salmonella test kits

27. Stomacher®.–Seward model 400 or equivalent.

28. Stomacher® type bags or equivalent.

29. Blender.–High speed (16,000 – 18,000 rpm) with sterile receptacle.

* Note: Please ensure that ample incubator space is available to accommodate the larger test portions. We estimate that each lab will need approximately 54 cubic feet (3 large incubators or an incubator room) to facilitate the testing of the 325 gram test portions. Overfilling the incubator with too many test samples may compromise the integrity of the results.

43