AOAC RI ERP Final Action Recommendations eBook

Dupont BAX Salmonella PTM Report Modification Approved 2012 / PTM Certification No. 081201 For Expert Review Panel Use Only Do Not Distribute

26

2. METHOD MODIFICATION AUTHORS

27

F. Morgan Wallace, Stephen Varkey, Daniel DeMarco, George Tice, Bridget Andaloro, Dawn Fallon, 28 Jeff Rohrbeck, Eugene Davis, Monica Tadler, Steven Hoelzer 29 DuPont Qualicon

30 31 32

3. SUBMITTING LABORATORY

33

DuPont Qualicon, P.O. Box 80400, Route 141 and Henry Clay Road, Wilmington, DE 19880 34 Phone 800-863-6842 or 302-695-5300 Fax 302-695-5301

35 36 37 38

4. SCOPE OF METHOD

39

1. Target organisms – Salmonella species.

40 41 42 43 44 45 46 47 48 49 50 51 52 53

2. Matrices – Specific matrices tested during the modification studies included ground beef,

cream cheese and dry pet food.

3. Performance claims – Sensitivity and specificity comparable to the appropriate reference

culture methods and equivalent or better than the unmodified method.

5. PRINCIPLE

AOAC INTERNATIONAL PCR amplification - The BAX® System uses the Polymerase Chain Reaction (PCR) to amplify a specific fragment of bacterial DNA, which is stable and unaffected by growth environment. The fragment is a genetic sequence that is unique to the genus Salmonella , thus providing a highly

reliable indicator that the organism is present. The BAX® System simplifies the PCR process by

combining the requisite primers, polymerase and nucleotides into a stable, dry, manufactured tablet

already packaged inside the PCR tubes. These tubes remain sealed after amplification, significantly