AOAC RI ERP Final Action Recommendations eBook

Dupont BAX Salmonella PTM Report Modification Approved 2012 / PTM Certification No. 081201 For Expert Review Panel Use Only Do Not Distribute

8. ADDITIONAL REAGENTS

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Lysis reagent – Using test kit reagents, pipette 150 µ L of protease into one 12-mL bottle of lysis

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buffer. Label bottle with the date prepared. Reagent will remain stable for up to two weeks if

stored at 2-8ºC.

9. APPARATUS

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Incubators – For maintaining media at 35 ± 1ºC and 39-42ºC.

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Stomacher – Seward model 400 or equivalent for mixing the sponge sample with enrichment

media.

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BAX ® System apparatus

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BAX® System Q7 cycler/detector with computer workstation

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BAX® System application software

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Cluster tubes with caps and racks for lysis

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Capping/de-capping tools – for removing and sealing cluster tube caps and PCR

tube caps without jarring the contents

Heating and cooling blocks with inserts – for maintaining lysis tubes at 37±2ºC,

95±3ºC, and 2-8°C.

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AOAC INTERNATIONAL Pipettes – for transferring reagents; two adjustable mechanical pipettes covering 20- 200 µ L and 5-50 µ L; one repeating pipette; and one multi-channel pipette covering 8 channels and 5-50 µ L. Pipettes should be calibrated to deliver required volumes

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within 10%.

Pipette tips with barriers: 0.5-250 µ L, 0.5-100 µ L extended barrier; 5 mL repeater

pipette tips