AOAC RI ERP Final Action Recommendations eBook

Dupont BAX Salmonella PTM Report Modification Approved 2012 / PTM Certification No. 081201 For Expert Review Panel Use Only Do Not Distribute

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PCR tube holders – for transferring a rack of tubes from the cooling block to the

cycler/detector

10. STANDARD REFERENCE CULTURES

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DuPont Qualicon Culture Collection (DD) - proprietary

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American Type Culture Collection (ATCC) - American Type Culture Collection (ATCC) -

www.atcc.org , American Type Culture Collection (ATCC), P.O. Box 1549, Manassas, VA

20108, USA.

11. MEDIA AND KIT COMPONENTS

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Items 1-4 are available as part of the BAX® System Real-Time PCR Assay for Salmonella .

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1. BAX® System PCR tubes (12 strips of 8 tubes containing one PCR tablet per tube)

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2. BAX® System lysis buffer (two bottles of 12 mL each);

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3. BAX® System protease (1 vial of 400 µ L)

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4. Flat optical caps (12 strips of 8 caps)

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5. Lactose broth – Dissolve 3 g of beef extract, 5 g of peptone and 5 g of lactose into 1 L of

distilled water. Dispense into containers and autoclave 15 minutes at 121ºC. Final pH is 6.9+/-0.2

6. BAX® System MP media – DuPont Qualicon catalog D12404925 (bulk powder), D12745725

AOAC INTERNATIONAL 7. Modified Tryptic Soy Broth containing glucose and 2 mg/L novobiocin (TSB + n), Difco (StatMedia)

catalog number 211825 or equivalent, BD, Franklin Lakes, NJ, 07417, USA

8. Buffered Peptone Water (BPW) – Oxoid CM 0509 or equivalent, Oxoid, Hampshire, United

Kingdom, RG24 8PW

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9. Brain Heart Infusion (BHI) broth – Oxoid CM 1032 or equivalent.