AOAC RI ERP Final Action Recommendations eBook

Dupont BAX Salmonella PTM Report Modification Approved 2012 / PTM Certification No. 081201 For Expert Review Panel Use Only Do Not Distribute

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10. Rappaport-Vassiliadis Soya Peptone (RVS), Selenite Cystine (SC), Tetrathionate-Hajna (TT-

Hajna) and Tetrathionate (TT) broths and Xylose Lysine Desoxycholate (XLD), Hektoen Enteric

(HE), Brilliant Green Sulfa (BGS) and Bismuth Sulfite (BS) agars and other appropriate

confirmatory media for reference method and alternate culture method confirmation.

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12. SAFETY PRECAUTIONS

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Kits — Reagents used in the BAX® System should pose no hazards when used as directed.

Dispose of lysate, PCR mixture and other waste according to your site practices.

Cycler/detector — Only qualified laboratory personnel should operate the cycler/detector. Do not

attempt to repair the instrument. Live power may still be available inside the unit even when a

fuse has blown or been removed. Refer to the BAX® System User Guide for maintenance

procedures when cleaning the unit or changing a fuse. The heating block can become hot enough

during normal operation to cause burns or cause liquids to boil. Wear safety glasses or other eye

protection at all times during operation.

Enrichment Broths — All enrichment broths may contain varying pathogens whether they contain

Salmonella or not and thus should be sterilized and disposed of using proper procedures

following any culture-based confirmatory steps.

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13. GENERAL PREPARATION / SAMPLE PREPARATION AND

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AOAC INTERNATIONAL RECOVERY Sample Preparation

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Ground beef, cream cheese and dry pet food samples were prepared for testing with either the

BAX® System method or the appropriate reference method as summarized in Table 1. Each sample

matrix was inoculated with a distinct Salmonella strain selected from the DuPont Qualicon Culture

Collection. A pure culture of each strain was inoculated into BHI broth and grown at 37°C overnight to