AOAC RI ERP Final Action Recommendations eBook

Dupont BAX Salmonella PTM Report PTM Certification No. 081201 (08/07/2012) For Expert Review Panel Use Only Do Not Distribute

12. STANDARD REFERENCE CULTURES

153

154

DuPont Qualicon Culture Collection (DD) - proprietary

155 156 157 158 159 160 161 162

American Type Culture Collection (ATCC) - American Type Culture Collection (ATCC) -

www.atcc.org , American Type Culture Collection (ATCC), P.O. Box 1549, Manassas, VA

20108, USA.

13. MEDIA AND KIT COMPONENTS

Items 1-4 are available as part of the BAX® System Real-Time PCR assay for Salmonella .

163

1. BAX® System PCR tubes (12 strips of 8 tubes containing one PCR tablet per tube)

164

2. BAX® System lysis buffer (two bottles of 12 mL each);

165

3. BAX® System protease (1 vial of 400 µ L)

166

4. Flat optical caps (12 strips of 8 caps)

167 168 169 170 171 172 173 174

5. Lactose broth – Dissolve 3 g of beef extract, 5 g of peptone and 5 g of lactose into 1 L of

distilled water. Dispense into containers and autoclave 15 minutes at 121ºC. Final pH is 6.9+/-0.2

6. BAX® System MP media – DuPont Qualicon catalog D12404925 (bulk powder), D12745725

(StatMedia)

7. Modified Tryptic Soy Broth containing glucose and 2 mg/L novobiocin (TSB + n), Difco

catalog number 211825 or equivalent, BD, Franklin Lakes, NJ, 07417, USA

AOAC INTERNATIONAL 8. Buffered Peptone Water (BPW) – Oxoid CM 0509 or equivalent, Oxoid, Hampshire, United Kingdom, RG24 8PW

175

9. Brain Heart Infusion (BHI) broth – Oxoid CM 1032 or equivalent.

176 177

10. Rappaport-Vassiliadis Soya Peptone (RVS), Selenite Cystine (SC), Tetrathionate-Hajna (TT-

Hajna) and Tetrathionate (TT) broths and Xylose Lysine Desoxycholate (XLD), Hektoen Enteric