AOAC RI ERP Final Action Recommendations eBook

Dupont BAX Salmonella PTM Report PTM Certification No. 081201 (08/07/2012) For Expert Review Panel Use Only Do Not Distribute

207 208 209 210 211 212 213 214 215 216 217 218 219

Sample Enrichment

Samples were prepared for testing with either the BAX® System method or the appropriate

reference culture method as summarized in Table 1. Meat, poultry, and pet food samples

were enriched in Buffered Peptone Water according to the USDA-FSIS and/or Compendium

methods for reference method testing, other sample types were enriched in LB as per the

FDA-BAM, while BAX® System samples were enriched in either BAX® System MP media,

TSB + novobiocin, and/or tested directly from the reference method enrichments. All

enrichments, without respect to presumptive result, subject to culture confirmation and

isolates were biochemically and serologically confirmed by the appropriate culture

methodology where suspect colonies were present, with at least one confirmed colony

obtained from an enrichment being counted as a positive sample.

Table 1. Matrices Tested, Reference Comparison Method, and Strains Used Matrix Inoculating Strain

Reference Method(s) MLG, HC

Ground Beef Chicken Rinse

DD13017 S . Heidelberg Naturally Occurring

MLG BAM BAM

Bagged Lettuce DD1261 S . Newport

Cream Cheese

DD586 S . Typhimurium

Dry Pet Food Environmental sponges Environmental sponges

DD13062 S . Tennessee

HC

DD12960 S . Senftenberg BAM

DD12960 S . Senftenberg MLG, HC

220 221 222 223

AOAC INTERNATIONAL MLG = USDA-FSIS Microbiological Laboratory Guidebook [4] HC = Health Canada Compendium of Analytical Methods [3]

BAM = U.S. FDA Bacteriological Analytical Manual [2]

224

225 226

Ground Beef – The BAX® System method was compared to the USDA-FSIS (USDA) and Health

Canada (HC) reference methods (which share the same primary enrichment media). A master