AOAC RI ERP Final Action Recommendations eBook
Table 2013.01B. Summary of results for the detection of Salmonella spp. in raw ground beef (375 g)
VIDAS SPT with traditional confirmation on BGSA and XLT4
VIDAS SPT with traditional confirmation on IBISA and ASAP b
VIDAS SPT with alternative confirmation on IBISA and ASAP c
Method a
Inoculation level Uninoculated Low
High
Uninoculated Low
High
Uninoculated
Low
High
Candidate
0/132
58/131
130/132
0/132
58/131
130/132
0/132
57/131
130/132
presumptive positive/total
samples analyzed Candidate
0.44 (+0.34, +0.55)
0.98 (+0.95, +1.00)
0.00 (0.00, +0.03)
0.44 (+0.33, +0.54)
0.98 (+0.965, +1.00)
0.00 (0.00, +0.03)
0.44 (+0.34, +0.55)
0.98 (+0.95, +1.00)
0.00 (0.00, +0.03)
presumptive POD (CP)
0.00 (0.00, +0.16) 0.00 (0.00, +0.16) 0.00 (0.00, +0.23)
0.49 (+0.43, +0.52) 0.10 (0.00, +0.27) 0.50 (+0.44, +0.52)
0.12 (+0.11, +0.16) 0.00 (0.00, +0.05) 0.12 (+0.11, +0.14)
0.00 (0.00, +0.16) 0.00 (0.00, +0.16) 0.00 (0.00, +0.23)
0.49 (+0.43, +0.52) 0.10 (0.00, +0.27) 0.50 (+0.44, +0.52)
0.12 (+0.11, +0.16) 0.00 (0.00, +0.05) 0.12 (+0.11, +0.14)
s
0.00 (0.00, +0.16) 0.00 (0.00, +0.16) 0.00 (0.00, +0.23)
0.49 (+0.44. +0.52) 0.09 (0.00, +0.26) 0.50 (+0.45, +0.52)
0.12 (+0.11, +0.16) 0.00 (0.00, +0.05) 0.12 (+0.11, +0.14)
d
r
s
e
L
s
f
R
P -value
1.0000
0.1551 58/131
0.5190 130/132
1.0000
0.1551 59/131
0.5190 130/132
1.0000
0.1906 58/131
0.5190 130/132
0/132
Candidate confirmed
0/132
0/132
positive/total samples analyzed
Candidate confirmed POD (CC)
0.00 (0.00, +0.03)
0.44 (+0.34, +0.55)
0.98 (+0.95, +1.00)
0.00 (0.00, +0.03)
0.45 (+0.35, +0.55)
0.98 (+0.95, +1.00)
0.00 (0.00, +0.03)
0.44 (+0.34, +0.55)
0.98 (+0.95, +1.00)
0.49 (+0.43, +0.52) 0.10 (0.00, +0.27) 0.50 (+0.45, +0.52)
0.12 (+0.11, +0.16) 0.00 (0.00, +0.05) 0.12 (0.11, +0.14)
0.00 (0.00, +0.16) 0.00 (0.00, +0.16) 0.00 (0.00, +0.23)
0.49 (+0.44, +0.52) 0.09 (0.00, +0.25) 0.50 (+0.45, +0.52)
0.12 (+0.11, +0.16) 0.00 (0.00, +0.05) 0.12 (+0.11, +0.14)
0.00 (0.00, +0.16) 0.00 (0.00, +0.16) 0.00 (0.00, +0.23)
0.49 (+0.43, +0.52) 0.10 (0.00, +0.27) 0.50 (+0.45, +0.52)
0.12 (+0.11, +0.16) 0.00 (0.00, +0.05) 0.12 (+0.11, +0.14)
0.00 (0.00, +0.16) 0.00 (0.00, +0.16) 0.00 (0.00, +0.23)
s
r
s
L
s
R
P -value Positive
1.0000
0.1551 57/132
0.5190 132/132
1.0000
0.2060 57/132
0.5190 132/132
1.0000
0.1551 54/132
0.5190 131/132
0/132
0/132
0/132
reference samples/total
samples analyzed
0.43 (+0.35, +0.52) 0.50 (+0.45, +0.52) 0.00 (0.00, +0.18) 0.50 (+0.45, +0.52) AOAC Research Institute Expert Review Panel Use Only 1.00 (+0.97, +1.00) 0.00 (0.00, +0.17) 0.00 (0.00, +0.17) 0.00 (0.00, +0.23) 0.00 (0.00, +0.03) 0.00 (0.00, +0.16) 0.00 (0.00, +0.16) 0.00 (0.00, +0.23) 0.43 (+0.35, +0.52) 0.50 (+0.45, +0.52) 0.00 (0.00, +0.18) 0.50 (+0.45, +0.52) 1.00 (+0.97, +1.00) 0.00 (0.00, +0.17) 0.00 (0.00, +0.17) 0.00 (0.00, +0.23) 0.00 (0.00, +0.03) 0.00 (0.00, +0.16) 0.00 (0.00, +0.16) 0.00 (0.00, +0.23) 0.41 (+0.32, +0.50) 0.49 (+0.44, +0.52) 0.05 (0.00, +0.22) 0.49 (+0.44, +0.52) 0.99 (+0.96, +1.00) 0.09 (+0.08, +0.16) 0.00 (0.00, +0.04) 0.09 (+0.08, +0.10)
Reference POD 0.00 (0.00, +0.03)
0.00 (0.00, +0.16) 0.00 (0.00, +0.16) 0.00 (0.00, +0.23)
s
r
s
L
s
R
P -value
1.0000
0.6261
1.0000
1.0000
0.6261
1.0000
1.0000
0.3313
0.4338
dLPOD (C vs R) 0.00 (–0.03, +0.03)
0.01 (–0.12, +0.15) 0.00 (–0.15, +0.15)
–0.02 (–0.05, +0.02) 0.00 (–0.04, +0.04)
0.00 (–0.03, +0.03) 0.00 (–0.03, +0.03)
0.02 (–0.18, +0.22) –0.01 (–0.15, +0.14)
–0.02 (–0.05, +0.02) 0.00 (–0.04, +0.04)
0.00 (–0.03, +0.03) 0.00 (–0.03, +0.03)
0.03 (–0.18, +0.24) –0.01 (–0.21, +0.23)
–0.01 (–0.05, +0.03) 0.00 (–0.04, +0.04)
dLPOD (CP vs CC)
0.00 (–0.03, +0.03)
a Results include 95% confidence intervals. b Traditional confirmation on ASAP/IBISA = secondary enrichments streaked onto IBISA and ASAP. c Alternative confirmation = direct streak of the primary enrichment onto IBISA and ASAP. d Repeatability standard deviation.
e Among-laboratory standard deviation. f Reproducibility standard deviation.
© 2013 AOAC INTERNATIONAL
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