AOAC RI ERP Final Action Recommendations eBook

AOAC EXPERT REVIEW PANEL FOR MICROBIOLOGY FOR FOODS AND ENVIRONMENTAL SURFACES AOAC Expert Review Panel Final Action Feedback

Figure 2. Tablets hydrated and loaded into the instrument 10 min after hydration.

II. The following change is requested during evaluation by the ERP for final action status:

After several incidents of very low target cell density enrichments being incorrectly called negative by the software, a change will be made in the BAX® software algorithm for this test kit to correct these erroneous calls. As a result of this change, two of 1938well calls associated with AOAC validation studies (both from the pre-collaborative study, one from the 25 g ground beef matrix and one from the 25 g ground turkey matrix) changed from correct negative calls to incorrect positive calls. The two amplification plots shown in Figures 3 and 4 are the PCR results from the samples for which algorithm calls changed with the new algorithm. Corrected statistical tables are presented for the two matrices in question. Tables 1 and 2 and 5 and 6 show the original data, while Tables 3 and 4 and 7 and 8 show the data when the results are analyzed with the new BAX® RT Salmonella algorithm. In both cases, the

Page 5 of 13