AOAC RI ERP Final Action Recommendations eBook

AOAC EXPERT REVIEW PANEL FOR MICROBIOLOGY FOR FOODS AND ENVIRONMENTAL SURFACES AOAC Expert Review Panel Final Action Feedback

Table 5. BAX® System Presumptive vs. Confirmed Results – Poultry and EggPresented Data Sample Type Media MPN a Test Portions BAX® Presumptive BAX® Confirmed X b POD CP c 95% CI f X b POD CC d 95% CI f

e

95% CI f

dPOD CP

Control

5

0 8

0

0

5 8

0

(0-0.43)

0 0

(-0.45, 0.45) (-0.14, 0.14)

0.63 (0.40, 1.0) 3.7 (2.0, 9.6)

(0.22, 0.61) (0.57, 1.0)

(0.22, 0.61) (0.57, 1.0)

Ground turkey (25 g)

20

0.40

0.40

BPW

5

5

1.0

5

1.0

0

(-0.45, 0.45)

a MPN = Most Probable Number based on the POD of reference method test portions using the AOAC MPN calculator (with 95% confidence interval) b X = Number of positive test portions c POD CP = Candidate method presumptive positive outcomes divided by the total number of trials d POD CC = Candidate method confirmed positive outcomes divided by the total number of trials e dPOD CP = Difference between the candidate method presumptive result and candidate method confirmed result POD values f 95% CI = If the confidence interval of a dPOD does not contain zero, then the difference is statistically significant at the 5% level

Table 6. BAX® System vs. Reference Method Results – Poultry and EggPresented Data Sample Type Media MPN a Test Portions BAX® Method X b POD C c 95% CI f X b POD CC d

e

95% CI f

Reference Method

dPOD C

95% CI f (0, 0.43)

(-0.45, 0.45) (-0.14, 0.14) (-0.45, 0.45)

Control

5

0

0

(0, 0.43) 0

0

0

Ground turkey (25 g)

0.63 (0.40, 1.0) 3.7 (2.0, 9.6)

(0.22, 0.61) (0.57, 1.0)

(0.22, 0.61)

BPW

20

8

0.40

8

0.40

0

5

5

1.0

5

1.0

(0.57, 1.0)

0

a MPN = Most Probable Number based on the POD of reference method test portions using the AOAC MPN calculator (with 95% confidence interval) b X = Number of positive test portions c POD C = Confirmed candidate method positive outcomes divided by the total number of trials d POD R = Confirmed reference method positive outcomes divided by the total number of trials e dPOD C = Difference between the candidate method and reference method POD values

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