AOAC RI ERP Final Action Recommendations eBook

FINAL (Version 4)

DuPont™ BAX ® System Real-Time PCR Assay for Salmonella : Collaborative Study

Table 1 - BAX® System vs. Reference Method Results for Orange Juice (Un-inoculated Control)

Candidate presumptive (CP)

Candidate confirmed (CC)

Candidate result (C)

Reference method (R)

C vs. R

MPN/ Test Portion

Statistic

Laboratory

dLPOD e (C,R)

dLPOD (CP,CC)

POD(CP) a

POD(CC) b

POD(C) c

POD(R) d

N

x

N

x

N

X

N

X

1

12

0

0.00

12

0

0.00

12

0

0.00

12

0

0.00

0.00

0.00

2 f

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

3 4 5 6 7 8 9

12 12 12 12 12 12 12 12 12 12 12 12 12

0 0 0 0 0 0 0 0 0 0 0 0 0 0

0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.02 0.00 0.00 0.15 0.00 0.00 0.15 0.00 0.00 0.21

12 12 12 12 12 12 12 12 12 12 12 12 12

0 0 0 0 0 0 0 0 0 0 0 0 0 0

0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.02 0.00 0.00 0.15 0.00 0.00 0.15 0.00 0.00 0.21

12 12 12 12 12 12 12 12 12 12 12 12 12

0 0 0 0 0 0 0 0 0 0 0 0 0 0

0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.02 0.00 0.00 0.15 0.00 0.00 0.15 0.00 0.00 0.21

12 12 12 12 12 12 12 12 12 12 12 12 12

0 0 0 0 0 0 0 0 0 0 0 0 0 0

0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.02 0.00 0.00 0.15 0.00 0.00 0.15 0.00 0.00 0.21

0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 -0.02 0.02

0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 -0.02 0.02

10 11 12 13 14 15 All

0.00 0.00 0.03

Estimate

168

168

168

168

LCL UCL

g

s r

LCL UCL

h

s L

LCL UCL

i

s R

UCL LCL

j

P T

1.0000

1.0000

1.0000

1.0000

1 2 3

a POD CP = Candidate method presumptive positive outcomes divided by the total number of trials, b POD CC Confirmed candidate method positive outcomes divided by the total number of trials, d POD R

= Candidate method confirmed positive outcomes divided by the total number of trials, c POD C = = Confirmed reference method positive outcomes divided by the total number of trials, e dLPOD is the

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