AOAC RI ERP Final Action Recommendations eBook

FINAL (Version 4)

DuPont™ BAX ® System Real-Time PCR Assay for Salmonella : Collaborative Study

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Table 2 - BAX® System vs. Reference Method Results for Orange Juice (Low Level Inoculum)

Candidate presumptive (CP)

Candidate confirmed (CC)

Candidate result (C)

Reference method (R)

C vs. R

MPN/ Test Portion

Statistic

Laboratory

dLPOD e (C,R)

dLPOD (CP,CC)

POD(CP) a

POD(CC) b

POD(C) c

POD(R) d

N

x

N

x

N

X

N

X

1

12

4

0.33

12

4

0.33

12

4

0.33

12

3

0.25

0.08

0.00

2 f

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

NA

3 4 5 6 7 8 9

12 12 12 12 12 12 12 12 12 12 12 12 12

6 7

0.50 0.58 0.83 0.50 0.50 0.67 0.33 0.67 0.75 0.50 0.58 0.50 0.58 0.56 0.48 0.64 0.50 0.45 0.52 0.00 0.00 0.18 0.50 0.45 0.52

12 12 12 12 12 12 12 12 12 12 12 12 12

6 7 9 6 6 8 4 8 9 5 7 6 7

0.50 0.58 075 0.50 0.50 0.67 0.33 0.67 0.75 0.42 0.58 0.50 0.58 0.55 0.47 0.62 0.50 0.45 0.52 0.00 0.00 0.17 0.50 0.45 0.52

12 12 12 12 12 12 12 12 12 12 12 12 12

6 7 9 6 6 8 4 8 9 5 7 6 7

0.50 0.58 075 0.50 0.50 0.67 0.33 0.67 0.75 0.42 0.58 0.50 0.58 0.55 0.47 0.62 0.50 0.45 0.52 0.00 0.00 0.17 0.50 0.45 0.52

12 12 12 12 12 12 12 12 12 12 12 12 12

6 5 8 6 5 6 7 8 5 6 7 6

0.50 0.42 0.67 0.50 0.42 0.50 0.58 0.67 0.42 0.50 0.58 0.50 0.83 0.52 0.45 0.60 0.50 0.45 0.52 0.00 0.00 0.18 0.50 0.45 0.52

0.00 0.16 0.08 0.00 0.08 0.17 -0.25 0.00 0.33 -0.08 0.00 0.00 -0.25 0.02 -0.08 0.13

0.00 0.00 0.08 0.00 0.00 0.00 0.00 0.00 0.00 0.08 0.00 0.00 0.00 0.01 -0.10 0.12

10

6 6 8 4 8 9 6 7 6 7

10 11 12 13 14 15 All

10 88

Estimate

168

94

168

92

168

92

168

0.37 0.16 0.88

LCL UCL

g

s r

LCL UCL

h

s L

LCL UCL

i

s R

UCL LCL

j

P T

0.4901

0.5903

0.5903

0.5021

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