AOAC-RI ERP Micro June 2016

OMAMAN-29 D/ PTM Validation Report 111501 OMA ERP - June 2016 ERP Use Only

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reference method confirmation procedure. There were 5 confirmed positives following the reference method. Detailed results of the POD analyses are presented in Tables 9 and 13.

4% Milk fat cottage cheese (25 g)

For the low inoculation level of the MDA 2 - Listeria assay, there were 12 presumptive positives and 12 confirmed positives following the AOAC 993.12 reference method confirmation procedure. There were 9 observed positives for the reference method. For the high inoculation level, there were 5 presumptive positives and 5 confirmed positives following the AOAC 993.12 reference method confirmation procedure. There were 5 confirmed positives following the reference method. Detailed results of the POD analyses are presented in Tables 9 and 13.

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Beef hot dog (25 g)

For the low inoculation level of the MDA 2 - Listeria assay, there were 4 presumptive positives and 5 confirmed positives following the USDA/FSIS MLG 8.09 reference method confirmation procedure, resulting in 1 false negative. There were 6 observed positives for the reference method. For the high inoculation level, there were 5 presumptive positives and 5 confirmed positives following the USDA/FSIS MLG 8.09 reference method confirmation procedure. There were 5 confirmed positives following the reference method. Detailed results of the POD analyses

are presented in Tables 9 and 13.

Stainless steel (sponge enriched in 225 mL)

Results were identical after 24 and 26 hours of sample enrichment. For the low inoculation level of the MDA 2 - Listeria assay, there were 5 presumptive positives and 5 confirmed positives following the USDA/FSIS MLG 8.09 reference method confirmation procedure. There were 8 observed positives for the reference method. For the high inoculation level, there were 5 presumptive positives and 5 confirmed positives following the USDA/FSIS MLG 8.09 reference method confirmation procedure. There were 5 confirmed positives following the reference method. Detailed results of the POD analyses are presented in Tables 9 and 13.

AOAC Research Institute Expert Review Panel Use Only

Sealed concrete (sponge enriched in 100 mL)

Results were identical after 24 and 26 hours of sample enrichment. For the low inoculation level of the MDA 2 - Listeria assay, there were 15 presumptive positives and 15 confirmed positives following the USDA/FSIS MLG 8.09 reference method confirmation procedure. There were 11 observed positives for the reference method. For the high inoculation level, there were 5 presumptive positives and 5 confirmed positives following the USDA/FSIS MLG 8.09 reference method confirmation procedure. There were 5 confirmed positives following the reference method. Detailed results of the POD analyses are presented in Tables 9 and 13.

Plastic (Enviroswab enriched in 10 mL)