AOAC-RI ERP Micro June 2016

OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol OMA ERP - June 2016 ERP Use Only

3M MDA 2 Listeria species and L. monocytogenes Collaborative Study OMA-2016-MONTH-XXX

Note condition of package on the data sheets and notify the Study Director if any packages 1 appear to have been compromised or if the food product does not arrive in the appropriate 2 condition. All refrigerated products should arrive cold to the touch and should be stored at 2-8 ° C 3 (refrigerated) until the day of analysis. 6 7 Collaborators will be provided with enough sample to test each matrix using the 3M MDA2 8 methods and the appropriate reference method. For raw chicken breast, collaborators will 9 receive approximately 60g. One test portion (25g) will be weighed and tested using the 3M 10 MDA2 - Listeria and 3M MDA2 - Listeria monocytogenes and the other test portion (25g) will be 11 weighed and tested using the reference method. For deli turkey they will receive two sets of test 12 portions: 36 x 125g (3M MDA 2 - Listeria monocytogenes and 3M MDA 2 - Listeria ) and 36 x 13 125g (USDA/FSIS MLG), for a total of 72 test portions. Please review the reference methods 14 carefully before initiating analysis. 15 16 One additional 60 g control (negative) test portion will be provided to each collaborator for each 17 matrix to determine the total plate count on the day of analysis using the 3M™ Petrifilm™ 18 Aerobic Count Plate method (OMA 990.12). One temperature control sample will also be 19 included in the shipment to document the temperature of the sample upon receipt. Record all 20 results on the data sheets provided. 4 5 Test Portion Analysis

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Reference Pre-enrichment

3M MDA2 Enrichment Demi Fraser Broth with FAC Demi Fraser Broth with FAC

3M MDA2 Test Kit

Food Type

Reference

Listeria & L. monocytogenes

USDA/FSIS MLG 8.09 USDA/FSIS MLG 8.09

Deli turkey

UVM

Raw chicken breast Listeria & L. monocytogenes *Tryptone Soya Broth with yeast plus acriflavine (9 mg/L), nalidixic acid (36 mg/L) and cycloheximide UVM 3M MDA2 - Listeria monocytogenes and MDA2 - Listeria To each 125g test portion, add 1125 mL Demi Fraser broth without ferric ammonium citrate (FAC). Homogenize each sample for two minutes ± 30 seconds AOAC Research Institute Expert Review Panel Use Only 1.2 Prepare the 3M MDS speed loader tray, chill block insert, heat block insert and instrument following the 3M MDA 2 - Listeria monocytogenes Instructions for 1.1 and incubate 24-30 h at 37 ± 1°C. (46 mg/L) 24 25 Deli turkey – use stomacher bag with filter for all 3M test portions. 1.0 23

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Use [IFU] and 3M Molecular Detection Instrument manual.

1.3 Equilibrate the LS tubes to room temperature (20-25 ºC) by setting LS tube overnight 16-18 hours. Or on the laboratory bench for at least 2 hours.

1.4 1.5

Invert the capped LS tubes to mix, up to 4 hours before use. Transfer 20 µL enriched sample into a LS tube.

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