AOAC-RI ERP Micro June 2016
OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol OMA ERP - June 2016 ERP Use Only
3M MDA 2 Listeria species and L. monocytogenes Collaborative Study OMA-2016-MONTH-XXX Appendix 8.3b – Study Flow Diagram: Raw Chicken Breast 1 2
Raw chicken breast- 25g
3MMDA 2- Listeria & 3MMDA 2- L. monocytogenes
USDA/FSIS MLG 8.09
25g + 475 mL prewarmed (37±1C ° ) Demi Fraser (no FAC), 28-32 hr at 37±1C °
25g + 225 mL UVM 20-26 hr at 30±2C °
Presumptive Result Using enriched sample, run 3M MDA Listeria monocytogenes test kit
MDA Confirmation Swab and streak to MOX, 24-28 hr hr at 35±2C °
MDA Confirmation Transfer 0.1 mL to Fraser broth, 24-28 hr hr at 35±2C °
Transfer 0.1 mL to Fraser broth, 24-28 hr hr at 35±2C °
Swab and streak to MOX, 24-28 hr hr at 35±2C °
Swab and streak to MOX, 24-28 hr hr at 35±2C °
Swab and streak to MOX, 24-28 hr hr at 35±2C °
Streak suspect colonies to TSA, 24 hr at 37±1 ° C
Streak suspect colonies to TSA, 24 hr at 37±1 ° C Streak suspect colonies to TSA, 24 hr at 37±1 ° C AOAC Research Institute Expert Review Panel Use Only Streak suspect colonies to TSA, 24 hr at 37±1 ° C
ID at least one colony using VITEK 2 GP or API Listeria
ID at least one colony using VITEK 2 GP or API Listeria
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