AOAC-RI ERP Micro June 2016

OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol OMA ERP - June 2016 ERP Use Only

several samples representative of the matrix, i.e. samples obtained from different origin, during 1 any validation period when adopting the 3M method or when testing new or unknown matrices 2 or matrices that have undergone raw material or process changes. 3 A matrix can be defined as a type of product with intrinsic properties such as composition and 4 process. Differences between matrices may be as simple as the effects caused by differences 5 in their processing or presentation for example, raw vs. pasteurized; fresh vs. dried, etc. 8 Store the 3M Molecular Detection Assay 2 - Listeria at 2-8°C. Do not freeze. Keep kit away from 9 light during storage. After opening the kit, check that the foil pouch is undamaged. If the pouch 10 is damaged, do not use. After opening, unused reagent tubes should always be stored in the re- 11 sealable pouch with the desiccant inside to maintain stability of the lyophilized reagents. Store 12 resealed pouches at 2-8°C for no longer than 60days. 13 14 Do not use 3M Molecular Detection Assay 2 - Listeria past the expiration date. Expiration date 15 and lot number are noted on the outside label of the box. After use, the enrichment medium and 16 the 3M Molecular Detection Assay 2 - Listeria tubes can potentially contain pathogenic 17 materials. When testing is complete, follow current industry standards for the disposal of 18 contaminated waste. Consult the Safety Data Sheet for additional information and local 19 regulations for disposal. 22 Follow all instructions carefully. Failure to do so may lead to inaccurate results. 23 Periodically decontaminate laboratory benches and equipment (pipettes, cap/decap tools, etc.) 24 with a 1- 5% (v:v in water) household bleach solution or DNA removal solution. 27 Table 2 presents guidance for the enrichment of food and environmental samples. It is the 28 user’s responsibility to validate alternate sampling protocols or dilution ratios to ensure this test 29 method meets the user’s criteria. 32 1. Allow the Demi-Fraser Broth enrichment medium (includes ferric ammonium citrate) to 33 equilibrate to ambient laboratory temperature. 34 AOAC Research Institute Expert Review Panel Use Only 6 7 STORAGE AND DISPOSAL 20 21 INSTRUCTIONS FOR USE 25 26 S AMPLE E NRICHMENT 30 31 Foods

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