AOAC-RI ERP Micro June 2016
OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol OMA ERP - June 2016 ERP Use Only
Detection Assay 2 – Listeria monocytogenes method is 1-5 colony forming units per
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validated test portion size (in Table 3).
Table 3. Enrichment protocols using Demi-Fraser Broth at 37 ± 1 °C according to AOAC Performance Tested SM Certificate #XXXXXX
Enrichment Broth Volume (mL)
Enrichment Time (hr)
Sample Matrix
Sample Size
Beef hot dogs, Queso Fresco, Vanilla Ice Cream, 4% Milk Fat Cottage Cheese, 3% chocolate whole milk, romaine lettuce, bagged raw spinach, cold smoked salmon
25 g
225
24-30
Raw chicken
25 g
475
28-32
Deli turkey
125 g
1125
24-30
Enough volume to allow melon to float
26-30
Cantaloupe
Whole melon
Stainless steel Sealed concrete
1 sponge
225
24-30
1 sponge AOAC Research Institute Expert Review Panel Use Only 100 Plastic 1 swab 10 24-30
Environmental samples:
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P REPARATION OF THE 3M™M OLECULAR D ETECTION S PEED L OADER T RAY 10
1. Wet a cloth with a 1-5% (v:v in water) household bleach solution and wipe the 3M™
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Molecular Detection Speed Loader Tray.
2. Rinse the 3M Molecular Detection Speed Loader Tray with water.
3. Use a disposable towel to wipe the 3M Molecular Detection Speed Loader Tray dry. 4. Ensure the 3M Molecular Detection Speed Loader Tray is dry before use.
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