AOAC-RI ERP Micro June 2016

OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol OMA ERP - June 2016 ERP Use Only

It is the user’s responsibility in selecting any test method or product to evaluate a sufficient 1 number of samples with the appropriate matrices and microbial challenges to satisfy the user 2 that the chosen test method meets the user’s criteria. 3 It is also the user’s responsibility to determine that any test methods and results meet its 4 customers’ and suppliers’ requirements. 5 As with any test method, results obtained from use of any 3M Food Safety product do not 6 constitute a guarantee of the quality of the matrices or processes tested. 7 To help customers evaluate the method for various food matrices, 3M has developed the 3M™ 8 Molecular Detection Matrix Control kit. When needed, use the Matrix Control (MC) to determine 9 if the matrix has the ability to impact the 3M Molecular Detection Assay 2 - Listeria 10 monocytogenes results. Test several samples representative of the matrix, i.e. samples 11 obtained from different origin, during any validation period when adopting the 3M method or 12 when testing new or unknown matrices or matrices that have undergone raw material or 13 process changes. 14 A matrix can be defined as a type of product with intrinsic properties such as composition and 15 process. Differences between matrices may be as simple as the effects caused by differences 16 in their processing or presentation for example, raw vs. pasteurized; fresh vs. dried, etc. 19 Store the 3M Molecular Detection Assay 2 - Listeria monocytogenes at 2-8°C. Do not freeze. 20 Keep kit away from light during storage. After opening the kit, check that the foil pouch is 21 undamaged. If the pouch is damaged, do not use. After opening, unused reagent tubes should 22 always be stored in the re-sealable pouch with the desiccant inside to maintain stability of the 23 lyophilized reagents. Store resealed pouches at 2-8°C for no longer than 60days. 24 25 Do not use 3M Molecular Detection Assay 2 - Listeria monocytogenes past the expiration date. 26 Expiration date and lot number are noted on the outside label of the box. After use, the 27 enrichment medium and the 3M Molecular Detection Assay 2 - Listeria monocytogenes tubes 28 can potentially contain pathogenic materials. When testing is complete, follow current industry 29 standards for the disposal of contaminated waste. Consult the Safety Data Sheet for additional 30 information and local regulations for disposal. 17 18 STORAGE AND DISPOSAL

AOAC Research Institute Expert Review Panel Use Only

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INSTRUCTIONS FOR USE 33 Follow all instructions carefully. Failure to do so may lead to inaccurate results. 34

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