AOAC SPADA Meeting

Publication

2018_10_17_SPADA_Brief

UNCLASSIFIED

21

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Proposed Modifications of Traditional Pathway for Development of a Molecular Assay

Reference Materials: live or inactivated organisms or genomic materials 1 prototype strain Multiple strains

Agnostic target/ ePCR validation/ranking PSET type analysis Synthetic metagenome assay design 1 strain or genomic material Pick only variant sequences plus target reads syn construct/surrogate for synthetic construct ePCR?

Courtesy: Hartman et al 2015. Clinical Chemistry 61:11 1391-1398. [Tim Minogue's group]

2018_10_17_SPADA_Brief

UNCLASSIFIED

22