AOAC SPADA Meeting

Assay Accuracies

Genus Bacillus Brucella

Inconclusive

Wrong Genus

Wrong Species

Correct

n

5% 3% 2% 9% 7% 8% 1% 0% 0%

4%

37% 21% 46% 12% 69% 41%

54% 256 62% 34 51% 191 78% 97 21% 29 14% 138 6% 104 10% 52 44% 32 36% 105 94% 17 17% 103 43% 132 55% 40 52% 127 45% 73 57% 14 67% 36 95% 20 0 0

15%

Burkholderia

2% 0% 3%

Francisella

Yersinia Bacillus Brucella MLST MIDI Ribotype Biolog Vitek Next Gen Sequencing Procedure • Extract DNA using EZ1 • Measure concentration with Qubit • Nextera DNA Flex Library prep kit • Run library prep on MiSeq using the v2  500 cycle kit • Run FASTQ files through EDGE  bioinformatics pipeline 37% ‐‐ ‐‐ ‐‐ ‐‐ Burkholderia 88% 87% 6% 4% Francisella Yersinia Bacillus Brucella 9% 47% 27% 23% 14% 6% 0% 2% 0% 8% 2% 0% Burkholderia 40% 51% 33% 18% 42% Francisella 6% 5% Yersinia Bacillus Brucella 28% ‐‐ ‐‐ ‐‐ ‐‐ Burkholderia 0% 4% 51% Francisella 21% 21% 25% 0% 6% 5% Yersinia Bacillus Brucella 3% 0% 0% ‐‐ ‐‐ ‐‐ ‐‐ Burkholderia 12% 0% 2% Francisella ‐‐ ‐‐ ‐‐ ‐‐ ‐‐ ‐‐ ‐‐ ‐‐ Yersinia

0

86% 103

0 0