AOAC SPADA Meeting

BURK001 Analysis • Repeated EDGE analysis with the following  input references

Reference

NCBI Accession

Percent Identity

Burkholderia cepacia  ATCC 25416 Burkholderia lata strain  383

CP007746, CP007748, CP007747, and  CP007745

99.98%

NC_007510, NC_007511, and NC_007509 97.69%

Burkholderia lata strain  FL‐7‐5‐30‐S1‐D0

NZ_CP013404, NZ_CP013405, and  NZ_CP013406

97.64%

• Only Burkholderia cepacia strain present in  EDGE database is GG4 – GG4 has two chromosomes – ATCC 25416 (BURK001) has three chromosomes  and a plasmid

Exempt BSAT

• Subspecies

– Francisella tularensis  subspecies novicida

• Virulence genes – Francisella tularensis  subspecies tularensis, SCHU S4∆clpB strain – Burkholderia pseudomallei  strain Bp82, a ΔpurM mutant of B.  pseudomallei strain 1026b deficient in purine biosynthesis • Strain – Coxiella burnetii Phase II, Nine Mile Strain, plaque purified clone 4 – Francisella tularensis biovar tularensis strain B‐38 (ATCC 6223) • Mobile elements – Bacillus anthracis  strains devoid of the plasmid pX02 – Yersinia pestis  strains devoid of the 75 kb low‐calcium response  (Lcr) virulence plasmid – Yersinia pestis strains which are Pgm‐ due to a deletion of a 102‐kb  region of the chromosome termed the pgm locus – Brucella abortus strain RB51 – Brucella abortus Strain 19