AOAC SPADA Meeting

Bacterial Verification

Where do Type strains come from? This does not mean that the Type Strain is the oldest evolutionary wise.  It refers to the date it is described Bacillus anthracis‐ ATCC 14578 ™ COHN (F.): Untersuchungen über Bakterien. Beitrage zur Biologie der Pflanzen , 1875 1 (Heft 2) , 1872, 1 , 127‐224. Brucella abortus‐ ATCC 23448™ MEYER (K.F.) and SHAW (E.B.): A comparison of the morphologic, cultural and biochemical characteristics of B. abortus and B. melitensis from cattle. Studies on the  genus Brucella nov. gen. Journal of Infectious Diseases , 1920, 27 , 173‐184. Brucella melitensis ‐ATCC 23456™ MEYER (K.F.) and SHAW (E.B.): A comparison of the morphologic, cultural and biochemical characteristics of B. abortus and B. melitensis from cattle. Studies on the  genus Brucella nov. gen. Journal of Infectious Diseases, 1920, 27, 173‐184. Brucella suis ‐ATCC 23444™ HUDDLESON (I.F.): The differentiation of the species of the genus Brucella. Michigan State College Agricultural Experimental Station Technical Bulletin, 1929, 100, 1‐16. Francisella tularensis   ATCC 6223™‐ 1)McCOY (G.W.) and CHAPIN (C.W.): Further observations on a plague‐like disease of rodents with a preliminary note on the causative  agent, Bacterium tularense. Journal of Infectious Diseases, 1912, 10, 61‐72. 2) DOROFEEV (K.A.): Classification of the causative agent of tularemia. Symposium Research Works  Institute Epidemiology and Microbiology Chita, 1947, 1, 170‐180 .  UBER (B.), ESCUDERO (R.), BUSSE (H.‐J.), SEIBOLD (E.), SCHOLZ (H.C.), ANDA (P.), KÄMPFER (P.) and SPLETTSTOESSER  (W.D.): Description of Francisella hispaniensis sp. nov., isolated from human blood, reclassification of Francisella novicida (Larson et al. 1955) Olsufiev et al. 1959 as Francisella  tularensis subsp. novicida comb. nov. and emended description of the genus Francisella. Int. J. Syst. Evol. Microbiol., 2010, 60, 1887‐1896. Yersinia pestis ATCC 19428™‐ 1 LEHMANN (K.B.) and NEUMANN (R.): Atlas und Grundriss der Bakteriologie und Lehrbuch der speziellen bakteriologischen Diagnostik, 1st ed., J.F. Lehmann,  München, 1896. 2 Van LOGHEM (J.J.): The classification of plague‐bacillus. Antonie van Leeuwenhoek Journal of Microbiology and Serology, 1944, 10, 15‐16.

Where do Type strains come from? Any other isolate that does not descend directly from that Type Strain should be considered another  “strain”.  The designation of the strain should follow the last name in the taxonomy: species, subspecies,  serotype, pathovar, etc. does not need to be officially adopted.      Bacterial Verification