AOAC SPADA Meeting

Bacterial Verification

Why verify? Safety/Legal

You could be in violation of regulations for proper shipping, storage and handling of the material. Name of the agent will determine the UN #, the Schedule B #, then the proper shipping containers and  hazardous materials declarations.  If it needs to be shipped across borders, the name will alert customs officials  to veterinary permits, plant pathogen permits,  infectious disease permits, etc.  If you get it wrong‐numerous agencies can claim jurisdiction over assessment of fines or worse.  Include‐ state  agencies, federal agencies such as Depts. of Transportation, Labor,  Commerce, Justice, HH&S and USDA.

These permits may be required for shipping this product: •This item is listed on the Commerce Control List of the U.S. Department of Commerce and is distributed only within the 50 United States. Distribution requires completion of a Customer Acceptance of Responsibility (CAR) for Commerce Control List Biologicals form. •Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

These permits may be required for shipping this product: • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.

These permits may be required for shipping this product: USDA APHIS PPQ Permit 526, plant pathogens permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. Isolation information for this item is required by USDA. Please check our website or contact Technical Service for assistance. Domestic isolates are easier to obtain than foreign isolates.

Bacterial Verification

Why verify?‐Professional Data generated with the wrong species will be rendered invalid.

Example‐ “I received a strain from a colleague.  Verified it was  G+ cocci by staining and did a  catalase test.  It was positive. I assumed it was  Staphylococcus and continued to use it.”  

Example‐ “The strain had some interesting properties. The DNA extracted from the culture was  positive for the plasmid of interest.  No microscopic examination was ever done that would have  shown a mix of G+ and G‐ organisms.”