AOAC SPADA Meeting

How is verification done in other kingdoms? Bacterial Verification

Cell line authentication: Species is verified by COI‐ ribosomal genes aligned with a public database “Barcode of Life” Strain‐verified by STR analysis of cell lines.  ATCC (and other collections) maintain a public database of STR  profiles of  human cell lines.  Service providers will analyze your cell line for the STR profile and provide 3 rd party verification that the STR matches (or does not match) database standard  for your cell line. Many journals and funding agencies now require 3 rd party authentication of cell lines.

Bacterial Verification

Considerations for Verification: The name‐ For example‐ will it be  Francisella tularensis  subsp. novicida or Francisella novicida  or  acknowledge all synonyms?

The sequence‐ if it needs to align with a reference sequence‐ Does the sequence needs to be identified  and secured?  

Description

Max score

Total score

Query cover E value Max ident

Clostridium acetobutylicum strain S512 16S ribosomal RNA gene, partial sequence 2242

2242 2242 2242

100% 0 100% 0 100% 0

100% 100% 100%

Bacillus anthracis strain R5‐331 16S ribosomal RNA gene, partial sequence Bacillus cereus strain OPP5 3‐2 16S ribosomal RNA gene, partial sequence

2242 2242