AOAC SPADA Meeting
Bacterial Verification
How many times do I need to verify?
Only the master cell bank needs to be verified‐ after distribution is not necessary? Upon receipt but not again? After a certain amount of passages? After changing growth media?
What happens at ATCC ? Bacterial Verification
Morphology/Microscopic observation VITEK ® MS Sequence analysis API ® ID Remel RapID™ Biolog Microbial ID VITEK ® 2 Serotyping Hundreds of biochemical tests Morphology/Microscopic observation Sequence analysis VITEK ® MS Morphology/Microscopic observation Sequence analysis VITEK ® MS API ® ID Remel RapID™ Biolog Microbial ID VITEK ® 2 Serotyping Hundreds of biochemical tests PCR
Did we receive the right thing?
Receive material
Grow & Preserve material
Is it pure?
Authenticate material
Authenticate the material
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10/15/2018
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