AOAC SPADA Meeting

Bacterial Verification

How many times do I need to verify?

Only the master cell bank needs to be verified‐ after distribution is not necessary? Upon receipt but not again? After a certain amount of passages? After changing growth media?

What happens at ATCC ? Bacterial Verification

 Morphology/Microscopic observation  VITEK ® MS  Sequence analysis  API ® ID   Remel RapID™  Biolog Microbial ID  VITEK ®  2  Serotyping  Hundreds of biochemical tests  Morphology/Microscopic observation  Sequence analysis  VITEK ® MS  Morphology/Microscopic observation  Sequence analysis  VITEK ® MS  API ® ID   Remel RapID™  Biolog Microbial ID  VITEK ®  2  Serotyping  Hundreds of biochemical tests  PCR

Did we receive the  right thing?

Receive material

Grow & Preserve  material

Is it pure?

Authenticate  material

Authenticate the  material

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10/15/2018