AOAC SPADA Meeting

3/11/2020

TOTAL NUMBER OF BACTERIAL GENOMES IN GENBANK VS THE PERCENTAGE OF COMPLETE BACTERIAL GENOMES AS A FUNCTION OF YEAR

 Databases are growing exponentially, but more incomplete genomes.  Use complete genome sequences for assay design  Use all sequences for assay validation

AOAC_SPADA_ISA_presentation

9

3/12/2020

9

SUMMARY OF RECOMMENDATIONS FOR PCR PRIMER DESIGN

• Full length genomes or genes • Near Neighbor sequences • Gather contaminating sequences

 In the publication, we provide reasons for these recommendations and give alternatives.

AOAC_SPADA_ISA_presentation

10

3/12/2020

10

5