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(18) Breadth of coverage .—Coverage for 95% of the genome should be at the minimum level or

higher depending on the expected application: 100% of target sequences should be at the minimum 237

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coverage or higher.

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(19) Cluster density .—The total length of all contigs or scaffolds should approximate the known

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genome size of the target organism (51).

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5 References

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1. Beck, L., Coates, S.G., Gee, J., Hadfield, T., Jackson, P., Keim, P., Lindler, L., Ostlund, V.E., Roberto, 243 F., Samuel, J., Sharma, S., Tallent, S., & Wagner, D.M. (2018) J. of AOAC Int . 101 , 1667-1707, 244 https://doi.org/10.1093/jaoac/101.6.1665 245

2. Valdivia-Granda, W.A. (2013) Virulence 4 , 745–751, doi: 10.4161/viru.26893 246 3. 247

Sichtig, H., Minogue, T., Yan, Y., Stefan, C., Hall, A., Tallon, L., Sadzewicz, L., Nadendla, S., Klimke,

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W., Hatcher, E., Shumway, M., Aldea, D.L., Allen, J., Koehler, J., Slezak, T., Lovell, S., Schoepp, R., &

Scherf, U. (2019) Nature Comm . 10 , 3313, https://doi.org/10.1038/s41467-019-11306-6

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4. Sakai, K., Takeda, M., Shimizu, S., Takahama, T., Yoshida, T., Watanabe, S., Iwasa, T., Yonesaka, K., 250

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Suzuki, S., Hayashi, H., Kawakami, H., Nonagase, Y., Tanaka, K., Tsurutani, J., Saigoh, K., Ito, A.,

Mitsudomi, T., Nakagawa, K., & Nishio, K. (2019) Sci. Rep . 9 , 11340,

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https://doi.org/10.1038/s41598-019-47673-9

5. Pandey, K.R., Maden, N., Poudel, B., Pradhananga, S., & Sharma, A.K. (2012) Genomics, Proteomics 254 & Bioinformatics 10 , 317–325, htto:/dx.do1.org/0.016/1.ob.2012.06.006 255 6. Katsoulakis, E., Duffy, J.E., Hintze, B., Spector, N.L., & Kelley, M.J. (2020) JCO Precis Oncol 4:212- 256

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221, DOI: 10.1200/PO.19.00118

7. Minogue, T.D., Koehler, J.W., Stefan, C.P., & Conrad, T.A. (2019) Clin. Chem . 65 , 383–392, 258 https://doi.org/10.1373/clinchem.2016.266536 259

AOAC Draft Standard – Version 09282022; Public Comment Revisions

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